Photosensitization of Colpoda inflata cysts bymeso-substituted cationic porphyrins
- 7 June 2002
- journal article
- research article
- Published by Springer Science and Business Media LLC in Photochemical & Photobiological Sciences
- Vol. 1 (8), 560-564
- https://doi.org/10.1039/b201267g
Abstract
Colpoda inflata cysts accumulate significantly large amounts of meso-substituted cationic porphyrins, including meso-tetrakis(N-methyl-4-pyridyl)porphine (TMP-C1) and its mono-N-methyldecyl (TMP-C10) and -tetradecyl (TMP-C14) analogues, upon incubation for 1 h with 1–48 µM porphyrin concentrations. All three porphyrins at concentrations greater than 10 µM exhibited a significant toxicity toward the cysts, in particular lowering the degree of excystment. Upon visible light irradiation of the porphyrin-loaded cysts, extensive photodamage was observed even in the presence of 1 µM porphyrin, as shown by light microscopy observations and counting of vegetative emerging cells. The presence of one decyl or tetradecyl hydrocarbon chain caused about 10-fold enhancement of the phototoxic effects.Keywords
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