Molecular organisation of the ice nucleation protein InaV from Pseudomonas syringae
Open Access
- 15 September 1997
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 414 (3), 590-594
- https://doi.org/10.1016/s0014-5793(97)01079-x
Abstract
A new ice nucleation gene from Pseudomonas syringae was isolated and overexpressed as a fully active protein in Escherichia coli in order to gain experimental data about the structure of ice nucleation proteins. No evidence of a signal sequence or secondary glycosylation was found. Differences in the extent of aggregation were shown to modulate the ice nucleation activity. The circular dichroism spectrum of the purified protein indicated the presence of β‐sheet structure. This finding supports a recently proposed hypothetical model for the structure of ice nucleation proteins, which provides a plausible explanation for their aggregation tendency.Keywords
This publication has 18 references indexed in Scilit:
- Kinetics of appearance and disappearance of classes of bacterial ice nuclei support an aggregation model for ice nucleus assemblyJournal of Bacteriology, 1993
- A Model of the Three-dimensional Structure of Ice Nucleation ProteinsJournal of Molecular Biology, 1993
- Bacterial Ice NucleationPublished by Elsevier BV ,1993
- Components of ice nucleation structures of bacteriaJournal of Bacteriology, 1991
- Localization of Ice Nucleation Activity and theiceCGene Product inPseudomonas syringaeandEscherichia coliMolecular Plant-Microbe Interactions®, 1989
- Size of bacterial ice-nucleation sites measured in situ by radiation inactivation analysisProceedings of the National Academy of Sciences of the United States of America, 1988
- Bacterial Ice Nucleation: Molecular Biology and ApplicationsBiotechnology and Genetic Engineering Reviews, 1987
- Some Basic Characteristics of Bacterial Freezing NucleiJournal of Applied Meteorology, 1981
- Quantitative Evaluation of Experimental Results an the Heterogeneous Freezing Nucleation of Supercooled LiquidsJournal of the Atmospheric Sciences, 1971
- Size Effect in Heterogeneous NucleationThe Journal of Chemical Physics, 1958