Application of high-throughput affinity-selection mass spectrometry for screening of chemical compound libraries in lead discovery
- 16 February 2007
- journal article
- Published by Informa Healthcare in Expert Opinion on Drug Discovery
- Vol. 2 (2), 285-294
- https://doi.org/10.1517/17460441.2.2.285
Abstract
High-throughput screening of chemical libraries for compounds that interfere with a particular molecular target is among the most powerful methodologies applied in lead discovery at present. In this review, the authors describe a label-free, homogeneous, affinity-selection-based technology developed at Novartis, termed SpeedScreen, which is compared with similar technologies used for high-throughput screening in the pharmaceutical and biotechnology industries. The focus at present of SpeedScreen is twofold: first, this technology is applied to orphan genomic targets and to those targets that are non-tractable by a functional assay; second, this technology is applied complementary to the well-established traditional methodologies for the screening of molecular targets. In summary, the authors discuss the value of affinity-selection-based high-throughput screening as a complementary technology to the common functional screening platforms and the benefits as well as the limitations of this new technology are outlined.Keywords
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