Identification and Characterization of a Phloem-Specific [beta]-Amylase
Open Access
- 1 November 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 109 (3), 743-750
- https://doi.org/10.1104/pp.109.3.743
Abstract
A monoclonal antibody, RS 5, was raised by injecting sieve elements isolated from tissue cultures of Streptanthus tortuosus (Brassicaceae) into BALB/c mice and screening resultant hybridoma supernatants for the labeling of phloem using immunofluorescence microscopy. The RS 5 monoclonal antibody identifies a 57-kD protein on immunoblots, which is present in phloem-forming tissue cultures of S. tortuosus but is absent in cultures that lack phloem. Purified 57-kD protein of S. tortuosus is demonstrated to be a phloem-specific [beta]-amylase. Partial peptide sequences of the 57-kD protein of S. tortuosus are shown to be 96% identical with the corresponding portions of a deduced sequence reported for a major form of [beta]-amylase in Arabidopsis thaliana. The RS 5 antibody cross-reacts with the major form of A. thaliana [beta]-amylase on immunoblots, and the antibody also binds to the sieve elements of A. thaliana using immunofluorescence microscopy. The results suggest that the major form of A. thaliana [beta]-amylase is a phloem-specific enzyme.Keywords
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