Heterodimer of two bHLH-PAS proteins mediates juvenile hormone-induced gene expression
- 27 December 2010
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 108 (2), 638-643
- https://doi.org/10.1073/pnas.1013914108
Abstract
Juvenile hormone (JH) plays crucial roles in many aspects of insect life. The Methoprene-tolerant (Met) gene product, a member of the bHLH-PAS family of transcriptional regulators, has been demonstrated to be a key component of the JH signaling pathway. However, the molecular function of Met in JH-induced signal transduction and gene regulation remains to be fully elucidated. Here we show that a transcriptional coactivator of the ecdysteroid receptor complex, FISC, acts as a functional partner of Met in mediating JH-induced gene expression. Met and FISC appear to use their PAS domains to form a dimer only in the presence of JH or JH analogs. In newly emerged adult female mosquitoes, expression of some JH responsive genes is considerably dampened when Met or FISC is depleted by RNAi. Met and FISC are found to be associated with the promoter of the early trypsin gene (AaET) when transcription of this gene is activated by JH. A juvenile hormone response element (JHRE) has been identified in the AaET upstream regulatory region and is bound in vitro by the Met-FISC complex present in the nuclear protein extracts of previtellogenic adult female mosquitoes. In addition, the Drosophila homologs of Met and FISC can also use this mosquito JHRE to activate gene transcription in response to JH in a cell transfection assay. Together, the evidence indicates that Met and FISC form a functional complex on the JHRE in the presence of JH and directly activate transcription of JH target genes.Keywords
This publication has 36 references indexed in Scilit:
- Paralogous Genes Involved in Juvenile Hormone Action in Drosophila melanogasterGenetics, 2010
- Coactivator recruitment: A new role for PAS domains in transcriptional regulation by the bHLH‐PAS familyJournal of Cellular Physiology, 2010
- bHLH-PAS family transcription factor methoprene-tolerant plays a key role in JH action in preventing the premature development of adult structures during larval–pupal metamorphosisMechanisms of Development, 2008
- Krüppel homolog 1 (Kr-h1) mediates juvenile hormone action during metamorphosis of Drosophila melanogasterMechanisms of Development, 2008
- Characterization of a juvenile hormone-regulated chymotrypsin-like serine protease gene in Aedes aegypti mosquitoInsect Biochemistry and Molecular Biology, 2008
- Identification and Characterization of a Juvenile Hormone Response Element and Its Binding ProteinsPublished by Elsevier BV ,2007
- Juvenile hormone resistance gene Methoprene-tolerant controls entry into metamorphosis in the beetle Tribolium castaneumProceedings of the National Academy of Sciences of the United States of America, 2007
- The Competence Factor βFtz-F1 Potentiates Ecdysone Receptor Activity via Recruiting a p160/SRC CoactivatorMolecular and Cellular Biology, 2006
- Interaction of bHLH-PAS proteins involved in juvenile hormone reception in DrosophilaBiochemical and Biophysical Research Communications, 2006
- Hormonal regulation and functional role of Drosophila E75A orphan nuclear receptor in the juvenile hormone signaling pathwayDevelopmental Biology, 2004