The role of the L3T4 molecule in mitogen and antigen-activated signal transduction
- 19 June 1987
- journal article
- Published by Elsevier BV in Cell
- Vol. 49 (6), 845-853
- https://doi.org/10.1016/0092-8674(87)90622-2
Abstract
We investigated the role of the L3T4 molecule in mitogen and antigen-initiated signal transduction in the L3T4(+) murine T cell hybridoma, 3DT52.5.9 and an L3T4(−) variant, 3DT52.5.24. Both Concanavalin A (Con A) and specific antigen stimulated increases in cytosolic-free calcium ([Ca 2+] i), phosphatidylinositol turnover, and interleukin-2 (IL-2) production in both cell lines. About 85% of the stimulated rise in [Ca 2+] i was from an extracellular source. Anti-L3T4 monoclonal antibody (MAb) inhibited 90% of antigen-and 50% of Con A-stimulated increases in [Ca 2+] i and IL-2 production but had no effect on the ability of either activation signal to stimulate phosphatidylinositol turnover in the parent L3T4(+) cells. Stimulus-response coupling in the L3T4(−) cells was unaffected by the MAb. The anti-L3T4-insensitive increase in [Ca 2+] i induced by Con A was inhibited by EGTA, suggesting that this mitogen also stimulated an influx of Ca 2+ via an additional transport mechanism distinct from that stimulated by antigen. The fact that anti-L3T4 antibodies inhibit antigen and Con A-stimulated Ca 2+ transport and IL-2 production without affecting phosphatidylinositol turnover suggests that L3T4 may play a critical role in modulating the activation of the T cell receptor-associated Ca 2+ transporter in T cell stimulus-response coupling.Keywords
Funding Information
- National Institutes of Health
- American Cancer Society (7-KOBCAOO156-03, CA-39264)
- Institut National Du Cancer
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