Additional Inhibitory Effect of Tea Extract on the Growth of Influenza A and B Viruses in MDCK Cells
Open Access
- 1 July 2002
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 46 (7), 491-494
- https://doi.org/10.1111/j.1348-0421.2002.tb02724.x
Abstract
It has been previously reported that green‐tea extract (GTE) inhibits the growth of influenza virus by preventing its adsorption. In this study, we further investigated whether GTE exerts an additional inhibitory effect on the acidification of intracellular compartments such as endosomes and lysosomes (referred to as ELS) and thereby inhibits the growth of influenza A and B viruses in Madin‐Darby canine kidney cells. The vital fluorescence microscopic study showed that GTE inhibited acidification of ELS in a concentration‐dependent manner. Moreover, the growth of influenza A and B viruses was equally inhibited when the cells were treated with GTE within as early as 5 to 15 min after infection, depending on the virus strains. The fact that (—)epigallocatechin (EGC), one of major catechin molecules in GTE, exerts the inhibitory effects on the acidification of ELS and virus growth in a manner similar to that of GTE strongly suggests that EGC is one of the active components in the extract.Keywords
This publication has 10 references indexed in Scilit:
- Inhibitory effect of Ephedrae herba, an oriental traditional medicine, on the growth of influenza A/PR/8 virus in MDCK cellsAntiviral Research, 1999
- Short communicationJournal of NeuroVirology, 1998
- Inhibitory effect of bafilomycin A1, a specific inhibitor of vacuolar-type proton pump, on the growth of influenza A and B viruses in MDCK cellsAntiviral Research, 1995
- Inhibition of the infectivity of influenza virus by tea polyphenolsAntiviral Research, 1993
- Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells.Journal of Biological Chemistry, 1991
- Inhibition of influenza virus infection by teaLetters in Applied Microbiology, 1990
- LysosomesPublished by Springer Science and Business Media LLC ,1989
- Antibody-mediated growth of influenza A NWS virus in macrophagelike cell line P388D1Journal of Virology, 1988
- Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assaysJournal of Immunological Methods, 1983
- Membrane fusion proteins of enveloped animal virusesQuarterly Reviews of Biophysics, 1983