Simultaneous Quantification of Ginsenosides in American Ginseng (Panax quinquefolium) Root Powder by Visible/Near-Infrared Reflectance Spectroscopy
- 19 June 1999
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 47 (7), 2771-2775
- https://doi.org/10.1021/jf9812477
Abstract
Near-infrared reflectance spectroscopy (NIRS) was examined as a possible alternative to high-performance liquid chromatography (HPLC) for the analysis of ginsenosides from American ginseng (Panax quinquefolium) root powder (n = 26). NIR spectra were collected over 400−2500 nm. For each sample and individual ginsenoside quantified by HPLC, spectral data were regressed against the chemical data to develop prediction equations. The spectral prediction equations produced high correlation coefficient (1-VR) values and low standard errors of cross validation (SECV) values for the determination of individual and total ginsenosides. The contents of individual ginsenosides, Rb1, Rb2, Rc, Rd, Re, Rg1, Ro, m-Rb1, m-Rb2, m-Rc, m-Rd, and total ginsenosides (X ± SECV) were (1.29 ± 0.18)%, (0.273 ± 0.096)%, (0.298 ± 0.052)%, (0.091 ± 0.027)%, (1.015 ± 0.114)%, (0.116 ± 0.018)%, (0.25 ± 0.040)%, (0.776 ± 0.116)%, (0.197 ± 0.074)%, (0.239 ± 0.083)%, (0.143 ± 0.042)%, and (4.393 ± 0.283)%, respectively. The (1-VR) values of cross validation were 0.877, 0.872, 0.955, 0.834, 0.899, 0.919, 0.325, 0.849, 0.902, 0.877, 0.871, and 0.963, respectively. Results indicated that the NIRS method could be used for the analysis of the major ginsenosides, Rb1, Re, and m-Rb1, as well as the total ginsenosides in American ginseng. Keywords: Panax quinquefolium; American ginseng; ginsenosides; quantification; HPLC; visible/NIR spectroscopyKeywords
This publication has 6 references indexed in Scilit:
- Panax ginseng pharmacology: A nitric oxide link?Biochemical Pharmacology, 1997
- The principles, practices and some future applications of near infrared spectroscopy for predicting the nutritive value of foods for animals and humansNutrition Research Reviews, 1997
- Use of Near-Infrared Spectroscopy to Evaluate an Active in a Film Coated TabletPharmaceutical Research, 1996
- A Comparative Study on Commercial Samples of Ginseng RadixPlanta Medica, 1995
- Simultaneous Analysis of Ginsenosides of Various Ginseng Radix by HPLCYAKUGAKU ZASSHI, 1995
- Determination of ginsenosides in ginseng crude extracts by high-performance liquid chromatographyJournal of Chromatography A, 1994