New technology for rapid molecular diagnosis of bloodstream infections
- 1 May 2010
- journal article
- Published by Taylor & Francis Ltd in Expert Review of Molecular Diagnostics
- Vol. 10 (4), 399-415
- https://doi.org/10.1586/erm.10.24
Abstract
Technologies for the correct and timely diagnosis of bloodstream infections are urgently needed. Molecular diagnostic methods have yet to have a major impact on the diagnosis of bloodstream infections; however, new methods are being developed that are beginning to address key issues. In this article, we discuss the key needs and objectives of molecular diagnostics for bloodstream infections and review some of the currently available methods and how these techniques meet key needs. We then focus on a new method that combines nucleic acid amplification with mass spectrometry in a novel approach to molecular diagnosis of bloodstream infections.Keywords
This publication has 63 references indexed in Scilit:
- Pathogen Profiling: Rapid Molecular Characterization of Staphylococcus aureus by PCR/Electrospray Ionization-Mass Spectrometry and Correlation with PhenotypeJournal of Clinical Microbiology, 2009
- Basic Concepts of Microarrays and Potential Applications in Clinical MicrobiologyClinical Microbiology Reviews, 2009
- Diagnosis of Bacteremia in Whole-Blood Samples by Use of a Commercial Universal 16S rRNA Gene-Based PCR and Sequence AnalysisJournal of Clinical Microbiology, 2009
- Identification of Methicillin-Resistant or Methicillin-Susceptible Staphylococcus aureus in Blood Cultures and Wound Swabs by GeneXpertJournal of Clinical Microbiology, 2009
- Molecular diagnosis of sepsis in neutropenic patients with haematological malignanciesJournal of Medical Microbiology, 2008
- Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia*Critical Care Medicine, 2008
- Multicenter Evaluation of the Candida albicans / Candida glabrata Peptide Nucleic Acid Fluorescent In Situ Hybridization Method for Simultaneous Dual-Color Identification of C. albicans and C. glabrata Directly from Blood Culture BottlesJournal of Clinical Microbiology, 2008
- A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samplesMedical Microbiology and Immunology, 2007
- Simultaneous Amplification and Identification of 25 Human Papillomavirus Types with Templex TechnologyJournal of Clinical Microbiology, 2006
- Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock*Critical Care Medicine, 2006