Interleukin 4 induces changes in the chromatin structure of the gamma 1 switch region in resting B cells before switch recombination.
Open Access
- 1 July 1990
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 172 (1), 375-378
- https://doi.org/10.1084/jem.172.1.375
Abstract
Interleukin 4 (IL-4) can induce the expression of IgG1 in sIgG- murine B cells stimulated with mitogens or through a cognate interaction with T helper (Th) cells. We have investigated the molecular basis for the IL-4-induced switch to IgG1 in lipopolysaccharide (LPS)-stimulated murine B cells and have previously shown that IL-4 induces transcription of the gamma 1 switch region before switch recombination. We now demonstrate that IL-4 induces a DNase I hypersensitive site at the 5' end of the gamma 1 switch region in resting B cells. LPS is not required, but it enhances induction. Hence, the interaction of IL-4 with its receptor results in increased accessibility of the gamma 1 switch region. The more open chromatin structure and increased transcriptional activity may be important in the selection of this region for switch recombination.This publication has 16 references indexed in Scilit:
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