Label‐free detection techniques for protein microarrays: Prospects, merits and challenges
- 18 February 2010
- journal article
- review article
- Published by Wiley in Proteomics
- Vol. 10 (4), 731-748
- https://doi.org/10.1002/pmic.200900458
Abstract
Protein microarrays, on which thousands of discrete proteins are printed, provide a valuable platform for functional analysis of the proteome. They have been widely used for biomarker discovery and to study protein–protein interactions. The accomplishments of DNA microarray technology, which had enabled massive parallel studies of gene expression, sparked great interest for the development of protein microarrays to achieve similar success at the protein level. Protein microarray detection techniques are often classified as being label‐based and label‐free. Most of the microarray applications have employed labelled detection such as fluorescent, chemiluminescent and radioactive labelling. These labelling strategies have synthetic challenges, multiple label issues and may exhibit interference with the binding site. Therefore, development of sensitive, reliable, high‐throughput, label‐free detection techniques are now attracting significant attention. Label‐free detection techniques monitor biomolecular interactions and simplify the bioassays by eliminating the need for secondary reactants. Moreover, they provide quantitative information for the binding kinetics. In this article, we will review several label‐free techniques, which offer promising applications for the protein microarrays, and discuss their prospects, merits and challenges.Keywords
This publication has 119 references indexed in Scilit:
- Spectral-domain optical coherence phase microscopy for label-free multiplexed protein microarray assayBiosensors and Bioelectronics, 2009
- Nanohole arrays of mixed designs and microwriting for simultaneous and multiple protein binding studiesBiosensors and Bioelectronics, 2009
- Measurement of enzyme kinetics and inhibitor constants using enthalpy arraysAnalytical Biochemistry, 2009
- Plasmonic Nanoholes in a Multichannel Microarray Format for Parallel Kinetic Assays and Differential SensingAnalytical Chemistry, 2009
- Detection of human proteins using arrayed imaging reflectometryBiosensors and Bioelectronics, 2008
- Enthalpy array analysis of enzymatic and binding reactionsAnalytical Biochemistry, 2008
- Peptide-coated nanotube-based biosensor for the detection of disease-specific autoantibodies in human serumBiosensors and Bioelectronics, 2008
- Development of Surface Plasmon Resonance Mass Spectrometry Array PlatformAnalytical Chemistry, 2007
- Antibody microarray‐based profiling of complex specimens: systematic evaluation of labeling strategiesProteomics, 2007
- Carbon Nanotube Amplification Strategies for Highly Sensitive Immunodetection of Cancer BiomarkersJournal of the American Chemical Society, 2006