Translating innate response into long‐lasting antibody response by the intrinsic antigen‐adjuvant properties of papaya mosaic virus
- 16 January 2008
- journal article
- Published by Wiley in Immunology
- Vol. 124 (2), 186-197
- https://doi.org/10.1111/j.1365-2567.2007.02753.x
Abstract
Identifying the properties of a molecule involved in the efficient activation of the innate and adaptive immune responses that lead to long-lasting immunity is crucial for vaccine and adjuvant development. Here we show that the papaya mosaic virus (PapMV) is recognized by the immune system as a pathogen-associated molecular pattern (PAMP) and as an antigen in mice (Pamptigen). A single immunization of PapMV without added adjuvant efficiently induced both cellular and specific long-lasting antibody responses. PapMV also efficiently activated innate immune responses, as shown by the induction of lipid raft aggregation, secretion of pro-inflammatory cytokines, up-regulation of co-stimulatory molecules on dendritic cells and macrophages, and long-lasting adjuvant effects upon the specific antibody responses to model antigens. PapMV mixed with Salmonella enterica serovar Typhi (S. typhi) outer membrane protein C increased its protective capacity against challenge with S. typhi, revealing the intrinsic adjuvant properties of PapMV in the induction of immunity. Antigen-presenting cells loaded with PapMV efficiently induced antibody responses in vivo, which may link the innate and adaptive responses observed. PapMV recognition as a Pamptigen might be translated into long-lasting antibody responses and protection observed. These properties could be used in the development of new vaccine platforms.Keywords
This publication has 42 references indexed in Scilit:
- Adjuvant-Enhanced Antibody Responses in the Absence of Toll-Like Receptor SignalingScience, 2006
- Adaptation of humoral memoryImmunological Reviews, 2006
- Translating Innate Immunity into Immunological Memory: Implications for Vaccine DevelopmentCell, 2006
- Effect of mutations K97A and E128A on RNA binding and self assembly of papaya mosaic potexvirus coat proteinThe FEBS Journal, 2005
- Salmonella porins induce a sustained, lifelong specific bactericidal antibody memory responseImmunology, 2005
- Maintenance of Serological Memory by Polyclonal Activation of Human Memory B CellsScience, 2002
- Effector and memory T-cell differentiation: implications for vaccine developmentNature Reviews Immunology, 2002
- ON IMMUNOLOGICAL MEMORYAnnual Review of Immunology, 1996
- Crystallization and Preliminary X-ray Analysis of Papaya Mosaic Virus Coat ProteinJournal of Molecular Biology, 1993
- Nucleotide Sequence of Papaya Mosaic Virus RNAJournal of General Virology, 1989