Contribution of morphology and membrane resistance to integration of fast synaptic signals in two thalamic cell types

Abstract

Thalamocortical cells (TCs) and interneurons (INs) in the lateral geniculate nucleus process visual information from the retina. The TCs have many short dendrites, whereas the INs have fewer and longer dendrites. Because of these morphological differences, it has been suggested that transmission of synaptic signals from dendritic synapses to soma is more efficient in TCs than in INs. However, a higher membrane resistance (R(m)) for the INs could, in theory, compensate for the attenuating effect of their long dendrites and allow distal synaptic inputs to significantly depolarize the soma. Compartmental models were made from biocytin filled TCs (n = 15) and INs (n = 3) and adjusted to fit the current- and voltage-clamp recordings from the individual cells. The confidence limits for the passive electrical parameters were explored by simulating the influence of noise, morphometric errors and non-uniform and active conductances. One of the useful findings was that R(m) was accurately estimated despite realistic levels of active conductance. Simulations to explore the somatic influence of dendritic synapses showed that a small (0.5 nS) excitatory synapse placed at different dendritic positions gave similar somatic potentials in the individual TCs, within the TC population and also between TCs and INs. A linear increase in the conductance of the synapse gave increases in somatic potentials that were more sublinear in INs than TCs. However, when the total synaptic conductance was increased by simultaneously activating many small, spatially distributed synapses, the INs converted the synaptic signals to soma potentials almost as efficiently as the TCs. Thus, INs can transfer fast synaptic signals to soma as efficiently as TCs except when the focal conductance is large.