Sperm-derived histones contribute to zygotic chromatin in humans
Open Access
- 31 March 2008
- journal article
- Published by Springer Science and Business Media LLC in BMC Developmental Biology
- Vol. 8 (1), 34
- https://doi.org/10.1186/1471-213x-8-34
Abstract
About 15% to 30% of the DNA in human sperm is packed in nucleosomes and transmission of this fraction to the embryo potentially serves as a mechanism to facilitate paternal epigenetic programs during embryonic development. However, hitherto it has not been established whether these nucleosomes are removed like the protamines or indeed contribute to paternal zygotic chromatin, thereby potentially contributing to the epigenome of the embryo. to clarify the fate of sperm-derived nucleosomes we have used the deposition characteristics of histone H3 variants from which follows that H3 replication variants present in zygotic paternal chromatin prior to S-phase originate from sperm. We have performed heterologous ICSI by injecting human sperm into mouse oocytes. Probing these zygotes with an antibody highly specific for the H3.1/H3.2 replication variants showed a clear signal in the decondensed human sperm chromatin prior to S-phase. In addition, staining of human multipronuclear zygotes also showed the H3.1/H3.2 replication variants in paternal chromatin prior to DNA replication. these findings reveal that sperm-derived nucleosomal chromatin contributes to paternal zygotic chromatin, potentially serving as a template for replication, when epigenetic information can be copied. Hence, the execution of epigenetic programs originating from transmitted paternal chromatin during subsequent embryonic development is a logical consequence of this observation.Keywords
This publication has 32 references indexed in Scilit:
- Epigenetic memory of an active gene state depends on histone H3.3 incorporation into chromatin in the absence of transcriptionNature, 2007
- Nucleosome stability mediated by histone variants H3.3 and H2A.ZGenes & Development, 2007
- Transmission of modified nucleosomes from the mouse male germline to the zygote and subsequent remodeling of paternal chromatinDevelopmental Biology, 2006
- Sperm Nuclear Histone to Protamine Ratio in Fertile and Infertile Men: Evidence of Heterogeneous Subpopulations of Spermatozoa in the EjaculateJournal of Andrology, 2006
- Histone H3 variants and their potential role in indexing mammalian genomes: The “H3 barcode hypothesis”Proceedings of the National Academy of Sciences of the United States of America, 2006
- Postmeiotic Sex Chromatin in the Male Germline of MiceCurrent Biology, 2006
- Cell Cycle regulations: The timing of pronuclear formation, DNA synthesis and cleavage in the human 1-cell embryoMolecular Human Reproduction, 1996
- DNA and total protamine masses in individual sperm from fertile mammalian subjectsCytometry, 1996
- Intracytoplasmic Sperm Injection in the Mouse1Biology of Reproduction, 1995
- Sequence-Specific Packaging of DNA in Human Sperm ChromatinScience, 1987