Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L.)
- 1 November 1994
- journal article
- Published by Springer Science and Business Media LLC in Plant Cell Reports
- Vol. 14 (1)
- https://doi.org/10.1007/bf00233289
Abstract
Somatic embryo formation occurred from immature zygotic embryos within ovules of stenospermocarpic seedless grapes (Vitis vinifera L.), when cultured for two months on liquid Emershad/Ramming medium. Somatic embryos continued to proliferate after excision and transfer to Emershad/Ramming medium supplemented with 1 μM benzylaminopurine and 0.65% TC agar. Plant development from somatic embryos was influenced by genotype, medium, phase (liquid, agar), stage (torpedo, mature) and their interactions. Optimal plant development occurred on Woody Plant Medium supplemented with 1.5% sucrose + 1 μM benzylaminopurine + 0.3% activated charcoal and 0.65% TC agar.Keywords
This publication has 4 references indexed in Scilit:
- The use of leaf GPI and IDH isozymes to examine the origin of polyembryony in cultured ovules of seedless grapePlant Cell Reports, 1989
- Embryogenic cell lines from somatic embryos of grape (Vitis vinifera L.)Plant Cell Reports, 1989
- Stimulation of somatic embryogenesis and plant regeneration from anther culture of Vitis vinifera cv. Cabernet-SauvignonPlant Cell Reports, 1986
- Dormancy in somatic embryos and seeds ofVitis: changes in endogenous abscisic acid during embryogeny and germinationPlanta, 1982