Advanced Multiplex PCR Assay for Differentiation of Brucella Species
Open Access
- 15 September 2011
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 77 (18), 6726-6728
- https://doi.org/10.1128/aem.00581-11
Abstract
Two new primer sets of a 766- and a 344-bp fragment were introduced into the conventional Bruce-ladder PCR assay. This novel multiplex PCR assay rapidly and concisely discriminates Brucella canis and Brucella microti from Brucella suis strains and also may differentiate all of the 10 Brucella species.Keywords
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