Technological applications of chlorophyll a fluorescence for the assessment of environmental pollutants
- 24 June 2011
- journal article
- review article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 401 (4), 1139-1151
- https://doi.org/10.1007/s00216-011-5166-1
Abstract
Chlorophyll a fluorescence has been extensively studied over the last few years. As demonstrated, this phenomenon is closely related to the state of photosystem II, which plays a leading role in the photosynthetic process, and therefore it has become a powerful tool to investigate this complex and any damage occurring in it as a result of physical or chemical stresses. This means that by using photosynthetic organisms as biological probes, one can consider chlorophyll a fluorescence as one of the techniques of choice to reveal the presence of some hazardous toxicants widely spread in the environment. Herbicides, pesticides, and heavy metals, whose concentration in water and food products is generally subject to extremely severe restrictions, are a concrete example of compounds detectable by chlorophyll a fluorescence. These dangerous substances react with the photosystem II, modifying the fluorescence emitted and giving responses which vary in a concentration-dependent manner. The possibility of performing easy, fast, and direct measurements of the fluorescence, even under light conditions, has opened new frontiers for the analysis in situ of pollutants. The aim of this review is to give an overview of the different techniques based on chlorophyll a fluorescence spectrometry, focusing in particular on those which represented the starting point for applications addressed to the assessment of toxic compounds in environmental samples.Keywords
This publication has 107 references indexed in Scilit:
- Structure‐based design of novel Chlamydomonas reinhardtii D1‐D2 photosynthetic proteins for herbicide monitoringProtein Science, 2009
- A rapid, non-invasive procedure for quantitative assessment of drought survival using chlorophyll fluorescencePlant Methods, 2008
- Plant tissue-and photosynthesis-based biosensorsBiotechnology Advances, 2008
- Chlorophyll Fluorescence: A Probe of Photosynthesis In VivoAnnual Review of Plant Biology, 2008
- Chlorophyll fluorescence imaging accurately quantifies freezing damage and cold acclimation responses in Arabidopsis leavesPlant Methods, 2008
- Control of the light harvesting function of chloroplast membranes: The LHCII‐aggregation model for non‐photochemical quenchingFEBS Letters, 2005
- A system for imaging variable chlorophyll fluorescence of aquatic phototrophsOphelia, 2004
- Development and performance characteristics of laser-induced fluorescence imaging lidar for forestry applicationsForest Ecology and Management, 2000
- Molecular mechanism of action of Pb2+ and Zn2+ on water oxidizing complex of photosystem IIFEBS Letters, 1994
- The Role of Chlorophyll Fluorescence in The Detection of Stress Conditions in PlantsC R C Critical Reviews in Analytical Chemistry, 1988