Effect of Calcium Phosphate Glass on Proliferation and Differentiation of MG-63 Cells in HA Scaffolds
- 15 March 2007
- journal article
- Published by Trans Tech Publications, Ltd. in Materials Science Forum
- Vol. 539-543, 731-736
- https://doi.org/10.4028/www.scientific.net/msf.539-543.731
Abstract
This study examined the possibility of synthesis of biphasic calcium phosphate by sintering a mixture of hydroxyapatite and calcium phosphate glass. The effect of the concentration of calcium phosphate glass in a mixture on the proliferation and differentiation of MG-63 preosteoblast-like cells in a hydtoxyapatite scaffold was investigated. The addition of 5 wt% of calcium phosphate glass significantly improved the level of attachment, proliferation and differentiation of MG-63 cells onto the hydroxyapatite scaffolds, particularly when the surface was modified with 2% bovine serum albumin (p<0.05). Under these conditions, type I collagen was expressed and the extracellular matrix was formed by 1 week, and the ALP gene was expressed at 4 weeks.Keywords
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