Comparison of prevalence, viral load, physical status and expression of human papillomavirus-16, -18 and -58 in esophageal and cervical cancer: a case-control study
Open Access
- 26 November 2010
- journal article
- Published by Springer Science and Business Media LLC in BMC Cancer
- Vol. 10 (1), 650
- https://doi.org/10.1186/1471-2407-10-650
Abstract
Background Human papillomavirus (HPV) infection is a major risk factor for the development of nearly all cases of cervical cancer worldwide. The presence of HPV DNA in cases of esophageal squamous-cell carcinoma (ESCC) has been reported repeatedly from Shantou, China, and other regions with a high incidence of esophageal carcinoma (EC). However, unlike in cervical squamous-cell carcinoma (CSCC), in ESCC, the characteristics of HPV are unclear. Thus, the role of high-risk HPV types in the carcinogenesis of ESCC remains uncertain. Methods Seventy cases of ESCC with 60 controls and 39 cases of CSCC with 54 controls collected from patients in Shantou region in China were compared for the distributions of HPV-16, -18 and -58; viral load; and viral integration using real-time PCR assay and HPV-16 expression using immunostaining. Results The detection rates and viral loads of HR-HPV infection were significantly lower in ESCC than in CSCC (50.0% vs. 79.48%, P = 0.005; 2.55 ± 3.19 vs. 361.29 ± 441.75, P = 0.002, respectively). The combined integration level of HPV-16, -18 and -58 was slightly lower in ESCC than in CSCC (P = 0.022). HPV-16 expression was detected in 59.26% of ESCC tissue and significantly associated with tumour grade (P = 0.027). Conclusions High levels of HR-HPV expression and integration may be an indicator of the risk of ESCC, at least for patients in the Shantou region of China. However, a relatively low HPV copy number and infection rate in ESCC is unlikely to play an essential a role in the carcinogenesis of ESCC as in cervical cancer. Factors other than HR-HPV infection may contribute to the carcinogenesis of ESCC.Keywords
This publication has 35 references indexed in Scilit:
- Detection of HPV DNA in esophageal cancer specimens from different regions and ethnic groups: a descriptive studyBMC Cancer, 2010
- Distribution of Six Oncogenic Types of Human Papillomavirus and Type 16 Integration Analysis in Chinese Women with Cervical Precancerous Lesions and CarcinomasTumor Biology, 2008
- Type-Dependent Integration Frequency of Human Papillomavirus Genomes in Cervical LesionsCancer Research, 2008
- Human papillomavirus type distribution in invasive cervical cancer and high‐grade cervical lesions: A meta‐analysis updateInternational Journal of Cancer, 2007
- Human papillomavirus in high- and low-risk areas of oesophageal squamous cell carcinoma in ChinaBritish Journal of Cancer, 2007
- Temporal trends of esophageal cancer during 1995–2004 in Nanao Island, an extremely high-risk area in ChinaEuropean Journal of Epidemiology, 2006
- Human Papillomavirus Type 16 Integration in Cervical Carcinoma In Situ and in Invasive Cervical CancerJournal of Clinical Microbiology, 2006
- A comprehensive analysis of HPV integration loci in anogenital lesions combining transcript and genome-based amplification techniquesOncogene, 2003
- Viral load of HPV in esophageal squamous cell carcinomaInternational Journal of Cancer, 2002
- Viral load of human papillomavirus and risk of CIN3 or cervical cancerThe Lancet, 2002