Quercetin Inhibits Left Ventricular Hypertrophy in Spontaneously Hypertensive Rats and Inhibits Angiotensin II-Induced H9C2 Cells Hypertrophy by Enhancing PPAR-γ Expression and Suppressing AP-1 Activity
Open Access
- 10 September 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (9), e72548
- https://doi.org/10.1371/journal.pone.0072548
Abstract
Quercetin is the most abundant flavonoid in fruit and vegetables and is believed to attenuate cardiovascular disease. We hypothesized that quercetin inhibits cardiac hypertrophy by blocking AP-1 (c-fos, c-jun) and activating PPAR-γ signaling pathways. The aim of this study was to identify the mechanism underlying quercetin-mediated attenuation of cardiac hypertrophy. Quercetin therapy reduced blood pressure and markedly reduced the ratio of left ventricular to body weight (LVW/BW) (P3H]leucine incorporation into H9C2 cells (64% reduction) and by the reduced hypertrophic surface area in H9C2 cells compared with the Ang II group (PPPPP<0.05, vs. SHRs). Furthermore, western blotting and real time-PCR analyses also showed that transfection with PPAR-γ siRNA significantly increased AP-1 signaling and reversed the effects of quercetin inhibition on mRNA expression levels of genes such as ANP and BNP in hypertrophic H9C2 cells. Our data indicate that quercetin may inhibit cardiac hypertrophy by enhancing PPAR-γ expression and by suppressing the AP-1 signaling pathway.Keywords
This publication has 37 references indexed in Scilit:
- Loss of AMP-Activated Protein Kinase-α2 Impairs the Insulin-Sensitizing Effect of Calorie Restriction in Skeletal MuscleDiabetes, 2012
- Quercetin Reduces Inflammatory Responses in LPS-Stimulated CardiomyoblastsOxidative Medicine and Cellular Longevity, 2012
- Protective effects of quercetin on liver injury induced by ethanolPharmacognosy Magazine, 2010
- Telmisartan-Induced Inhibition of Vascular Cell Proliferation Beyond Angiotensin Receptor Blockade and Peroxisome Proliferator-Activated Receptor-γ ActivationHypertension, 2009
- Modulation of the caveolin-3 localization to caveolae and STAT3 to mitochondria by catecholamine-induced cardiac hypertrophy in H9c2 cardiomyoblastsExperimental & Molecular Medicine, 2009
- Modulation of the caveolin-3 and Akt status in caveolae by insulin resistance in H9c2 cardiomyoblastsExperimental & Molecular Medicine, 2005
- Quercetin glucuronide prevents VSMC hypertrophy by angiotensin II via the inhibition of JNK and AP-1 signaling pathwayBiochemical and Biophysical Research Communications, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Suppression of inducible cyclooxygenase and nitric oxide synthase through activation of peroxisome proliferator‐activated receptor‐γ by flavonoids in mouse macrophagesFEBS Letters, 2001
- Important Role of Angiotensin II–Mediated c-Jun NH 2 -Terminal Kinase Activation in Cardiac Hypertrophy in Hypertensive RatsHypertension, 2000