The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow
- 22 March 2010
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Organic & Biomolecular Chemistry
- Vol. 8 (10), 2419-2424
- https://doi.org/10.1039/b924100k
Abstract
Through the preparation of a novel controlled pore glass-poly(pyrrole) material we have developed a conducting support that is not only suitable for the co-immobilisation of enzymes and co-factors, but also enables the facile electrochemical regeneration of the co-factor during a reaction. Employing the selective reduction of (rac)-2-phenylpropionaldehyde to (S)-phenyl-1-propanol as a model, we have demonstrated the successful co-immobilisation of the HLADH enzyme and co-factor NAD(H); with incorporation of the material into a continuous flow reactor facilitating the in situ electrochemical regeneration of NAD(H) for in excess of 100 h. Using this approach we have developed a reagent-less, atom efficient system applicable to the cost-effective, continuous biosynthesis of chiral compounds.Keywords
This publication has 18 references indexed in Scilit:
- Evaluation of the Heterogeneously Catalyzed Strecker Reaction Conducted Under Continuous FlowEuropean Journal of Organic Chemistry, 2008
- Enzymatic synthesis of a series of alkyl esters using novozyme 435 in a packed-bed, miniaturized, continuous flow reactorBiocatalysis and Biotransformation, 2008
- Conductive Sol-Gel Hybrid Materials for Novel Cofactor Regeneration in BiocatalysisSolid State Phenomena, 2007
- [3 + 2] Cycloaddition of acetylenes with azides to give 1,4-disubstituted 1,2,3-triazoles in a modular flow reactorOrganic & Biomolecular Chemistry, 2007
- Cofactor regeneration for sustainable enzymatic biosynthesisBiotechnology Advances, 2007
- The use of electroosmotic flow as a pumping mechanism for semi-preparative scale continuous flow synthesisChemical Communications, 2006
- Integrated Microfluidics for Parallel Screening of an In Situ Click Chemistry LibraryAngewandte Chemie-International Edition, 2006
- Immobilization of invertase and glucose oxidase in conducting H-type polysiloxane/polypyrrole block copolymersReactive and Functional Polymers, 2003
- Porous conducting membranes based on polypyrrole–PMMA compositesSynthetic Metals, 1999
- A Simple Procedure for Covalent Immobilization of NADH in a Soluble and Enzymically Active FormJBIC Journal of Biological Inorganic Chemistry, 1980