Encoded Silica Colloidal Crystal Beads as Supports for Potential Multiplex Immunoassay
- 5 February 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 80 (5), 1598-1605
- https://doi.org/10.1021/ac702249a
Abstract
We developed a new kind of suspension array for multiplexed immunoassays using silica colloidal crystal beads (SCCBs) as coding carriers. The monodisperse and size-controlled SCCBs were fabricated by a microfluidic device. Calcination was employed to improve the mechanical stability and lower the fluorescent background of the SCCBs. Immobilization of protein molecules on the surface of the SCCBs through chemical bonds was studied, and the modification condition was optimized to increase the detection sensitivity. Results indicated that the SCCBs as supports were more sensitive (0.92 ng/mL IgG) than the glass beads (27 ng/mL IgG) and the planar carriers (140 ng/mL IgG). A multiplex immunoassay showed the flexibility and feasibility of SCCBs array in clinical applications.Keywords
This publication has 30 references indexed in Scilit:
- Porous SiO2Interferometric Biosensor for Quantitative Determination of Protein Interactions: Binding of Protein A to Immunoglobulins Derived from Different SpeciesAnalytical Chemistry, 2006
- Duplexed sandwich immunoassays on a fiber-optic microarrayAnalytica Chimica Acta, 2006
- Quantum dot bioconjugates for imaging, labelling and sensingNature Materials, 2005
- Fabrication of Multicolor‐Encoded Microspheres by Tagging Semiconductor Nanocrystals to Hydrogel SpheresAdvanced Materials, 2005
- Application of microchip assay system for the measurement of C-reactive protein in human salivaLab on a Chip, 2005
- Encoding microcarriers: present and future technologiesNature Reviews Drug Discovery, 2002
- A Microchip-Based Multianalyte Assay System for the Assessment of Cardiac RiskAnalytical Chemistry, 2002
- Towards the DRED of Resin-Supported Combinatorial Libraries: A Non-Invasive Methodology Based on Bead Self-Encoding and Multispectral ImagingAngewandte Chemie, 2000
- Euclidean shape-encoded combinatorial chemical librariesProceedings of the National Academy of Sciences of the United States of America, 2000
- A Homogeneous and Multiplexed Immunoassay for High-Throughput Screening Using Fluorometric Microvolume Assay TechnologyAnalytical Biochemistry, 1999