Unique cholesteryl glucosides in Helicobacter pylori: composition and structural analysis
Open Access
- 1 September 1995
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 177 (18), 5327-5333
- https://doi.org/10.1128/jb.177.18.5327-5333.1995
Abstract
A chloroform-methanol-extracted lipid of Helicobacter pylori was studied. Three kinds of glycolipids, accounting for about 25% (wt/wt) of the total lipid, were detected and identified to be cholesteryl glucosides. The structures of two of them were determined to be cholesteryl-alpha-D-glucopyranoside and cholesteryl-6-O-tetrade-canoyl-alpha-D-glucopyranoside, and the plausible structure of the third one was identified as cholesteryl-6-O-phosphatidyl-alpha-D-glucopyranoside. Cholesteryl glucosides are very rare in animals and bacteria. Furthermore, those in H. pylori had an alpha-glycosidic linkage, which is rather unusual for natural glycosides, and a phosphate-linked cholesteryl glycoside like the cholesteryl-6-O-phosphatidyl-alpha-D-glucopyranoside has not been reported previously. As the cholesterol glucosides were detected in strains obtained from diverse geographical locations, the presence of cholesteryl glucosides in H. pylori is a very unique and a characteristic feature of the species. These findings add a new facet to the physiology and biochemistry, especially the cholesterol and glucose metabolism, of H. pylori. Furthermore, the cholesteryl glucosides of H. pylori showed hemolytic activities.Keywords
This publication has 28 references indexed in Scilit:
- Glucose metabolism by Helicobacter pyloriMicrobiology, 1994
- Glucose utilization and lactate production by Helicobacter pyloriJournal of General Microbiology, 1993
- Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pyloriThe Lancet, 1993
- Structures and properties of acyl diglucosylcholesterol and galactofuranosyl diacylglycerol from Acholeplasma axanthumBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
- UNIDENTIFIED CURVED BACILLI ON GASTRIC EPITHELIUM IN ACTIVE CHRONIC GASTRITISThe Lancet, 1983
- Cholesteryl glucoside in Candida bogoriensisBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
- Alteration of the phospholipid composition of Staphylo-coccus aureus cultured in medium containing NaClBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1972
- Cholesterol glucoside in tobaccoPhytochemistry, 1970
- Determination of glycerol in phosphatidesBiochimica et Biophysica Acta, 1962
- Phosphatides of human blood cells and their role in spherocytosisBiochimica et Biophysica Acta, 1961