In Vivo Metabolic Profiles of Panax notoginseng Saponins Mediated by Gut Microbiota in Rats
- 25 May 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 68 (25), 6835-6844
- https://doi.org/10.1021/acs.jafc.0c01857
Abstract
Panax notoginseng saponins (PNS) are the major health-beneficial components of P. notoginseng with very low oral bioavailability, which could be bio-transformed by gut microbiota in vitro. However, in vivo biotransformation of PNS mediated by gut microbiota is not well-known. This study aimed to characterize the in vivo metabolic profiles of PNS mediated by gut microbiota. The saponins and yielded metabolites in rat feces were identified and relatively quantified by UPLC-QTOF-MS/MS. Seventy-three PNS metabolites had been identified in normal control group, but only eleven PNS metabolites were determined in pseudo germ-free (GF) group. In addition, the main biotransformation pathway of PNS metabolism was hydrolytic and dehydration reactions. The results indicated that significant metabolic difference was observed between normal control group and pseudo GF group, while gut microbiota played a profound role on the biotransformation of PNS in vivo.Keywords
Funding Information
- Natural Science Foundation of Hunan Province (2020JJ1307)
- National Natural Science Foundation of China (81903784)
This publication has 27 references indexed in Scilit:
- Qualitative and quantitative analysis of the saponins in Panax notoginseng leaves using ultra-performance liquid chromatography coupled with time-of-flight tandem mass spectrometry and high performance liquid chromatography coupled with UV detectorJournal of Ginseng Research, 2018
- Gut microbiota in the pharmacokinetics and colonic deglycosylation metabolism of ginsenoside Rb1 in rats: Contrary effects of antimicrobials treatment and restraint stressChemico-Biological Interactions, 2016
- Traditional uses, botany, phytochemistry, pharmacology and toxicology of Panax notoginseng (Burk.) F.H. Chen: A reviewJournal of Ethnopharmacology, 2016
- Phytochemistry and Anticancer Potential of NotoginsengThe American Journal of Chinese Medicine, 2016
- Pharmacokinetics, bioavailability, and metabolism of Notoginsenoside Fc in rats by liquid chromatography/electrospray ionization tandem mass spectrometryJournal of Pharmaceutical and Biomedical Analysis, 2015
- Effects of broad-spectrum antibiotics on the metabolism and pharmacokinetics of ginsenoside Rb1: A study on rats׳ gut microflora influenced by lincomycinJournal of Ethnopharmacology, 2014
- Saponins ofPanax notoginseng: chemistry, cellular targets and therapeutic opportunities in cardiovascular diseasesExpert Opinion on Investigational Drugs, 2014
- Protective Effects of Panax Notoginseng Saponins on Cardiovascular Diseases: A Comprehensive Overview of Experimental StudiesEvidence-Based Complementary and Alternative Medicine, 2014
- Absorption and Disposition of Ginsenosides after Oral Administration of Panax notoginseng Extract to RatsDrug Metabolism and Disposition, 2009
- Pharmacological activity of sanchi ginseng (Panax notoginseng)Journal of Pharmacy and Pharmacology, 2006