High-density oligonucleotide arrays

Abstract

We are attempting to produce high-density arrays of surface-bound oligonucleotides for hybridization experiments. Our approach is to mechanically miniaturize conventional DNA synthesis chemistry. Ink-jet printer heads are used to deliver small drops of reagents to a chemically modified silicon dioxide surface, where they react to synthesize DNA. The drops are confined to small areas by a pattern of hydrophobic surface modifications, thus allowing the formation of high-density arrays without mixing between adjacent drops.