Analyzing Binding Data
Open Access
- 1 July 2010
- journal article
- unit
- Published by Wiley in Current Protocols in Neuroscience
- Vol. 52 (1), 7.5.1-7.5.65
- https://doi.org/10.1002/0471142301.ns0705s52
Abstract
Measuring the rate and extent of radioligand binding provides information on the number of binding sites, and their affinity and accessibility of these binding sites for various drugs. This unit explains how to design and analyze such experiments. Curr. Protoc. Neurosci. 52:7.5.1-7.5.65. © 2010 by John Wiley & Sons, Inc.Keywords
This publication has 9 references indexed in Scilit:
- A Simple Theoretical Model for Fluorescence Polarization Binding Assay DevelopmentSLAS Discovery, 2006
- Identification of Small-Molecule Inhibitors of RGS4 Using a High-Throughput Flow Cytometry Protein Interaction AssayMolecular Pharmacology, 2006
- Principles of Fluorescence SpectroscopyPublished by Springer Science and Business Media LLC ,2006
- Fluorescent BODIPY-GTP Analogs: Real-Time Measurement of Nucleotide Binding to G ProteinsAnalytical Biochemistry, 2001
- Quantitative Fluorescence Resonance Energy Transfer Measurements Using Fluorescence MicroscopyBiophysical Journal, 1998
- LIGAND: A versatile computerized approach for characterization of ligand-binding systemsAnalytical Biochemistry, 1980
- Equilibrium binding of [3H]tubocurarine and [3H]acetylcholine by torpedo postsynaptic membranes: stoichiometry and ligand interactionsBiochemistry, 1979
- Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reactionBiochemical Pharmacology, 1973
- A graphic method for the determination and presentation of binding parameters in a complex systemAnalytical Biochemistry, 1967