Structural Insights into the Assembly of the Type III Secretion Needle Complex
- 5 November 2004
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 306 (5698), 1040-1042
- https://doi.org/10.1126/science.1102610
Abstract
Type III secretion systems (TTSSs) mediate translocation of virulence factors into host cells. We report the 17-angstrom resolution structures of a central component of Salmonella typhimurium TTSS, the needle complex, and its assembly precursor, the bacterial envelope–anchored base. Both the base and the fully assembled needle complex adopted multiple oligomeric states in vivo, and needle assembly was accompanied by recruitment of the protein PrgJ as a structural component of the base. Moreover, conformational changes during needle assembly created scaffolds for anchoring both PrgJ and the needle substructure and may provide the basis for substrate-specificity switching during type III secretion.Keywords
This publication has 13 references indexed in Scilit:
- Synthesis and Localization of the Salmonella SPI-1 Type III Secretion Needle Complex Proteins PrgI and PrgJJournal of Bacteriology, 2003
- Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation processThe EMBO Journal, 2002
- SalmonellaInteractions with Host Cells: Type III Secretion at WorkAnnual Review of Cell and Developmental Biology, 2001
- Genetic Analysis of Assembly of theSalmonella entericaSerovar Typhimurium Type III Secretion-Associated Needle ComplexJournal of Bacteriology, 2001
- Assembly and Function of Type III Secretory SystemsAnnual Review of Microbiology, 2000
- Contribution of Salmonella typhimurium type III secretion components to needle complex formationProceedings of the National Academy of Sciences of the United States of America, 2000
- Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion systemProceedings of the National Academy of Sciences of the United States of America, 2000
- Type III Secretion Machines: Bacterial Devices for Protein Delivery into Host CellsScience, 1999
- Supramolecular Structure of the Salmonella typhimurium Type III Protein Secretion SystemScience, 1998
- Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells.Proceedings of the National Academy of Sciences of the United States of America, 1989