Using a Commercial DiversiLab Semiautomated Repetitive Sequence-Based PCR Typing Technique for Identification of Escherichia coli Clone ST131 Producing CTX-M-15
- 1 April 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 47 (4), 1212-1215
- https://doi.org/10.1128/jcm.02265-08
Abstract
A study was designed to evaluate the ability of the DiversiLab fingerprinting kit, a type of repetitive element PCR (rep-PCR), to identify Escherichia coli clone ST131 producing β-lactamase CTX-M-15. A set of 53 nonduplicate isolates of extended-spectrum β-lactamase-producing E. coli underwent rep-PCR, pulsed-field gel electrophoresis, and multilocus sequence typing. The DiversiLab system successfully identified E. coli clone ST131 producing CTX-M-15 and provides a simple standardized typing protocol for monitoring the spread of this clone.Keywords
This publication has 11 references indexed in Scilit:
- UK epidemic Escherichia coli strains A-E, with CTX-M-15 -lactamase, all belong to the international O25:H4-ST131 cloneJournal of Antimicrobial Chemotherapy, 2008
- European Emergence of Ciprofloxacin-Resistant Escherichia coli Clonal Groups O25:H4-ST 131 and O15:K52:H1 Causing Community-Acquired Uncomplicated CystitisJournal of Clinical Microbiology, 2008
- Extended-spectrum β-lactamase-producing Enterobacteriaceae: an emerging public-health concernThe Lancet Infectious Diseases, 2008
- Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15Emerging Infectious Diseases, 2008
- Turkey: a further country concerned by community-acquired Escherichia coli clone O25-ST131 producing CTX-M-15Journal of Antimicrobial Chemotherapy, 2008
- Intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15Journal of Antimicrobial Chemotherapy, 2007
- Molecular Epidemiology of CTX-M-Producing Escherichia coli in the Calgary Health Region: Emergence of CTX-M-15-Producing IsolatesAntimicrobial Agents and Chemotherapy, 2007
- Rapid Detection and Identification of Bacterial Pathogens Using Novel Molecular Technologies: Infection Control and BeyondClinical Infectious Diseases, 2007
- Multilocus Sequence Typing of BacteriaAnnual Review of Microbiology, 2006
- Establishment of a Universal Size Standard Strain for Use with the PulseNet Standardized Pulsed-Field Gel Electrophoresis Protocols: Converting the National Databases to the New Size StandardJournal of Clinical Microbiology, 2005