Regeneration in Alfalfa Tissue Culture
- 1 November 1985
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 79 (3), 584-589
- https://doi.org/10.1104/pp.79.3.584
Abstract
The composition of phosphorus-containing compounds of intact lyophilized alfalfa tissue has been determined, in part, by solid-state (31)P nuclear magnetic resonance. The tissue (Medicago sativa L., cv Regen S; and some of its crosses) was grown in culture under both nonregenerating and regenerating conditions, the latter enhanced by the addition of specific amino acids. Analysis of the (31)P nuclear magnetic resonance spectra shows that regeneration is favored when metabolism occurs without the production of a low average intracellular pH.Keywords
This publication has 9 references indexed in Scilit:
- Combining solid-state and solution-state 31P NMR to study in vivo phosphorus metabolism.Proceedings of the National Academy of Sciences of the United States of America, 1985
- Investigation of mineral phases of bone by solid-state phosphorus-31 magic-angle sample-spinning nuclear magnetic resonanceBiochemistry, 1984
- Solid-state phosphorus-31 nuclear magnetic resonance studies of synthetic solid phases of calcium phosphate: potential models of bone mineralBiochemistry, 1984
- Mechanisms of cytoplasmic pH regulation in hypoxic maize root tips and its role in survival under hypoxia.Proceedings of the National Academy of Sciences of the United States of America, 1984
- A 31P Nuclear Magnetic Resonance Study of Intracellular pH of Plant Cells Cultivated in Liquid MediumPlant Physiology, 1982
- Ion Balance, Uptake, and Transport Processes in N2-Fixing and Nitrate- and Urea-Dependent Soybean PlantsPlant Physiology, 1982
- N.m.r. studies of metabolism in perfused organsPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1980
- Studies of acidosis in the ischaemic heart by phosphorus nuclear magnetic resonanceBiochemical Journal, 1979
- Muscular fatigue investigated by phosphorus nuclear magnetic resonanceNature, 1978