Dexamethasone Mediated Stabilization of Insulin Receptor mRNA
- 1 January 1994
- journal article
- research article
- Published by Informa UK Limited in Journal of Receptor Research
- Vol. 14 (5), 297-305
- https://doi.org/10.3109/10799899409066038
Abstract
To determine the mechanism of glucocorticoid mediated enhancement of insulin receptor (IR) gene expression, we cotransfected a glucocorticoid receptor expression vector and a plasmid containing a reporter gene driven by an MMTV or IR promoter into COS 7 cells. Dexamethasone (Dex) increased MMTV promoter activity by 100% but had no effect on IR promoter activity. In the glucocorticoid responsive breast cancer cell line, MCF-7, Dex increased IR mRNA by 60%, and increased the IR mRNA half-life from approximately 6hrs to >24hrs. No glucocorticoid responsive element could be located in the insulin receptor 3′ untranslated region. Glucocorticoids stabilize IR mRNA.Keywords
This publication has 7 references indexed in Scilit:
- Cytoplasmic regulation of mRNA function: The importance of the 3′ untranslated regionCell, 1993
- Effects of Media Conditions, Insulin, and Dexamethasone on Insulin-Receptor mRNA and Promoter Activity in HepG2 CellsDiabetes, 1991
- Do the poly(A) tail and 3′ untranslated region control mRNA translation?Cell, 1990
- High Concentrations of Estrogen Stabilize Vitellogenin mRNA against Cytoplasmic Degradation but Physiological Concentrations do notMolecular Endocrinology, 1990
- 3' noncoding region of phosphoenolpyruvate carboxykinase mRNA contains a glucocorticoid-responsive mRNA-stabilizing element.Proceedings of the National Academy of Sciences of the United States of America, 1989
- Regulation of Insulin-Receptor mRNA Levels by GlucocorticoidsDiabetes, 1987
- Glucocorticoids enhance stability of human growth hormone mRNA.Molecular and Cellular Biology, 1987