Complete genome sequence of a serotype 11A, ST62 Streptococcus pneumoniaeinvasive isolate
Open Access
- 1 February 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Microbiology
- Vol. 11 (1), 25
- https://doi.org/10.1186/1471-2180-11-25
Abstract
Background: Streptococcus pneumoniae is an important human pathogen representing a major cause of morbidity and mortality worldwide. We sequenced the genome of a serotype 11A, ST62 S. pneumoniae invasive isolate (AP200), that was erythromycin-resistant due to the presence of the erm(TR) determinant, and carried out analysis of the genome organization and comparison with other pneumococcal genomes. Results: The genome sequence of S. pneumoniae AP200 is 2,130,580 base pair in length. The genome carries 2216 coding sequences (CDS), 56 tRNA, and 12 rRNA genes. Of the CDSs, 72.9% have a predicted biological known function. AP200 contains the pilus islet 2 and, although its phenotype corresponds to serotype 11A, it contains an 11D capsular locus. Chromosomal rearrangements resulting from a large inversion across the replication axis, and horizontal gene transfer events were observed. The chromosomal inversion is likely implicated in the rebalance of the chromosomal architecture affected by the insertions of two large exogenous elements, the erm(TR)-carrying Tn1806 and a functional prophage designated ϕSpn_200. Tn1806 is 52,457 bp in size and comprises 49 ORFs. Comparative analysis of Tn1806 revealed the presence of a similar genetic element or part of it in related species such as Streptococcus pyogenes and also in the anaerobic species Finegoldia magna, Anaerococcus prevotii and Clostridium difficile. The genome of ϕSpn_200 is 35,989 bp in size and is organized in 47 ORFs grouped into five functional modules. Prophages similar to ϕSpn_200 were found in pneumococci and in other streptococcal species, showing a high degree of exchange of functional modules. ϕSpn_200 viral particles have morphologic characteristics typical of the Siphoviridae family and are capable of infecting a pneumococcal recipient strain. Conclusions: The sequence of S. pneumoniae AP200 chromosome revealed a dynamic genome, characterized by chromosomal rearrangements and horizontal gene transfers. The overall diversity of AP200 is driven mainly by the presence of the exogenous elements Tn1806 and ϕSpn_200 that show large gene exchanges with other genetic elements of different bacterial species. These genetic elements likely provide AP200 with additional genes, such as those conferring antibiotic-resistance, promoting its adaptation to the environment.Keywords
This publication has 72 references indexed in Scilit:
- Serotype specific invasive capacity and persistent reduction in invasive pneumococcal diseaseVaccine, 2010
- Genomewide Screening for Novel Genetic Variations Associated with Ciprofloxacin Resistance in Bacillus anthracisAntimicrobial Agents and Chemotherapy, 2010
- A New Pneumococcal Serotype, 11E, Has a Variably InactivatedwcjEGeneThe Journal of Infectious Diseases, 2010
- Genetic Elements Responsible for Erythromycin Resistance in StreptococciAntimicrobial Agents and Chemotherapy, 2009
- Whole-Genome Pyrosequencing of an Epidemic Multidrug-Resistant Acinetobacter baumannii Strain Belonging to the European Clone II GroupAntimicrobial Agents and Chemotherapy, 2008
- Effects of Routine Infant Vaccination With the 7-Valent Pneumococcal Conjugate Vaccine on Nasopharyngeal Colonization With Streptococcus pneumoniae in Children in Calgary, CanadaThe Pediatric Infectious Disease Journal, 2008
- New Genetic Element Carrying the Erythromycin Resistance Determinant erm (TR) in Streptococcus pneumoniaeAntimicrobial Agents and Chemotherapy, 2008
- Complete Genome Sequence of Finegoldia magna, an Anaerobic Opportunistic PathogenDNA Research, 2008
- Streptococcus pneumoniae colonisation: the key to pneumococcal diseaseThe Lancet Infectious Diseases, 2004
- Improved microbial gene identification with GLIMMERNucleic Acids Research, 1999