Intranasal administration of Lactobacillus rhamnosus GG protects mice from H1N1 influenza virus infection by regulating respiratory immune responses
Open Access
- 29 March 2010
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 50 (6), 597-602
- https://doi.org/10.1111/j.1472-765x.2010.02844.x
Abstract
Aims: To investigate whether intranasal Lactobacillus administration protects host animals from influenza virus (IFV) infection by enhancing respiratory immune responses in a mouse model. Methods and Results: After 3 days of intranasal exposure to Lactobacillus rhamnosus GG (LGG), BALB/c mice were infected with IFV A/PR/8/34 (H1N1). Mice treated with LGG showed a lower frequency of accumulated symptoms and a higher survival rate than control mice (P < 0·05). The YAC-1 cell-killing activity of lung cells isolated from mice treated with LGG was significantly greater than those isolated from control mice (P < 0·01). Intranasal administration of LGG significantly increased mRNA expression of interleukin (IL)-1β, tumour necrosis factor (TNF) and monocyte chemotactic protein (MCP)-1 (P < 0·01). Conclusions: These results suggest that intranasal administration of LGG protects the host animal from IFV infection by enhancing respiratory cell-mediated immune responses following up-regulation of lung natural killer (NK) cell activation. Significance and Impact of Study: We have demonstrated that probiotics might protect host animals from viral infection by stimulating immune responses in the respiratory tract.Keywords
This publication has 9 references indexed in Scilit:
- Lactobacillus GG in the prevention of gastrointestinal and respiratory tract infections in children who attend day care centers: A randomized, double-blind, placebo-controlled trialClinical Nutrition, 2010
- Clinical Indications for Probiotics: An OverviewClinical Infectious Diseases, 2008
- Synergistic effect of intratumoral IL-12 and TNF-α microspheres: systemic anti-tumor immunity is mediated by both CD8+ CTL and NK cellsSurgery, 2007
- Essential Roles of Monocytes in Stimulating Human Peripheral Blood Mononuclear Cells with Lactobacillus casei To Produce Cytokines and Augment Natural Killer Cell ActivityClinical and Vaccine Immunology, 2006
- Interleukin-12 is involved in the enhancement of human natural killer cell activity byLactobacillus caseiShirotaClinical and Experimental Immunology, 2006
- Reduction of Influenza Virus Titer and Protection against Influenza Virus Infection in Infant Mice FedLactobacillus caseiShirotaClinical and Vaccine Immunology, 2004
- Effect of long term consumption of probiotic milk on infections in children attending day care centres: double blind, randomisedBMJ, 2001
- Effect of Intranasal Administration ofLactobacillus caseiShirota on Influenza Virus Infection of Upper Respiratory Tract in MiceClinical and Diagnostic Laboratory Immunology, 2001
- Induction of natural killer cell migration by monocyte chemotactic protein−1, −2 and −3European Journal of Immunology, 1994