Growth factor‐stimulated MAP kinase induces rapid retrophosphorylation and inhibition of MAP kinase kinase (MEK1)
- 13 June 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 346 (2-3), 299-303
- https://doi.org/10.1016/0014-5793(94)00475-7
Abstract
The MAP kinase module (Raf/MAPKKK‐MAPKK‐MAPK) has been shown to be sequentially activated after mitogenic stimulation. Here we demonstrate, by site directed mutagenesis, that MAPK is able to retrophosphorylate its own activator, MAPKK, on two threonine residues Thr‐292 and Thr‐386 in vitro, and that these sites are also phosphorylated in vivo. A comparison of the kinetics of serum‐mediated activation of a wild‐type MAPKK and of a mutant unable to undergo phosphorylation by MAPK suggests that this retrophosphorylation may be involved in a negative feedback control of the cascade in vivo.This publication has 18 references indexed in Scilit:
- MAP kinase kinase kinase, MAP kinase kinase and MAP kinaseCurrent Opinion in Genetics & Development, 1994
- Inhibition of the EGF-Activated MAP Kinase Signaling Pathway by Adenosine 3′,5′-MonophosphateScience, 1993
- MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivoCell, 1993
- Mitogen-activated protein kinases p42mapk and p44mapk are required for fibroblast proliferation.Proceedings of the National Academy of Sciences of the United States of America, 1993
- Functional expression and growth factor activation of an epitope-tagged p44 mitogen-activated protein kinase, p44mapk.Molecular Biology of the Cell, 1992
- Site-directed mutagenesis of virtually any plasmid by eliminating a unique siteAnalytical Biochemistry, 1992
- [11] Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose platesMethods in Enzymology, 1991
- A specific mutation abolishing Na+/H+ antiport activity in hamster fibroblasts precludes growth at neutral and acidic pH.Proceedings of the National Academy of Sciences of the United States of America, 1984
- The structure of an antigenic determinant in a proteinCell, 1984
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970