Molecular Characterization of Fluoroquinolone-Resistant Aeromonas spp. Isolated from Imported Shrimp
Open Access
- 15 November 2012
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 78 (22), 8137-8141
- https://doi.org/10.1128/aem.02081-12
Abstract
Sixty-three nalidixic acid-resistant Aeromonas sp. isolates were obtained from imported shrimp. Phylogenetic analysis of gyrB sequences indicated that 18 were A. enteropelogenes , 26 were A. caviae , and 19 were A. sobria . Double missense mutations in the quinolone resistance-determining region (QRDR) of gyrA at codon 83 (Ser→Val/Ile) and codon 92 (Leu→Met) coupled with a point mutation of parC at codon 80 (Ser→Ile/Phe) conferred high levels of quinolone resistance in the isolates. A majority of A. enteropelogenes and A. caviae strains harbored toxin genes, whereas only a few A. sobria strains harbored these genes. The fluoroquinolone-resistant Aeromonas spp. exhibited higher cytotoxicity than fluoroquinolone-sensitive, virulent Aeromonas spp. to rat epithelial cells.Keywords
This publication has 26 references indexed in Scilit:
- The Type II Secretion System Is Essential for Erythrocyte Lysis and Gut Colonization by the Leech Digestive Tract Symbiont Aeromonas veroniiApplied and Environmental Microbiology, 2011
- Detection and characterization of virulence genes and integrons in Aeromonas veronii isolated from catfishFood Microbiology, 2010
- A Type VI Secretion System Effector Protein, VgrG1, from Aeromonas hydrophila That Induces Host Cell Toxicity by ADP Ribosylation of ActinJournal of Bacteriology, 2010
- Shrimp farming in China: Operating characteristics, environmental impact and perspectivesOcean & Coastal Management, 2007
- Secreted enzymes of AeromonasFEMS Microbiology Letters, 2006
- Mechanisms of quinolone resistance in Escherichia coli and Salmonella: Recent developmentsInternational Journal of Antimicrobial Agents, 2005
- A Type III Secretion System Is Required for Aeromonas hydrophila AH-1 PathogenesisInfection and Immunity, 2004
- Phylogenetic analysis of members of the genus Aeromonas based on gyrB gene sequencesInternational Journal of Systematic and Evolutionary Microbiology, 2003
- Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protectionJournal of Antimicrobial Chemotherapy, 2003
- Aeromonas spp as a potential cause of diarrhoea in children.Journal of Clinical Pathology, 1992