Monoclonal Behavior of Molecularly Imprinted Polymer Nanoparticles in Capillary Electrochromatography
- 13 March 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 80 (8), 2881-2887
- https://doi.org/10.1021/ac070038v
Abstract
A new approach based on miniemulsion polymerization is demonstrated for synthesis of molecularly imprinted nanoparticles (MIP-NP; 30−150 nm) with “monoclonal” binding behavior. The performance of the MIP nanoparticles is characterized with partial filling capillary electrochromatography, for the analysis of rac-propranolol, where (S)-propranolol is used as a template. In contrast to previous HPLC and CEC methods based on the use of MIPs, there is no apparent tailing for the enantiomer peaks, and baseline separation with 25 000−60 000 plate number is achieved. These effects are attributed to reduction of the MIP site heterogeneity by means of peripheral location of the core cross-linked NP and to MIP-binding sites with the same ordered radial orientation. This new MIP approach is based on the substitution of the functional monomers with a surfactant monomer, sodium N-undecenoyl glycinate (SUG) for improved inclusion in the MIP-NP structure and to the use of a miniemulsion in the MIP-NP synthesis. The feasibility of working primarily with aqueous electrolytes (10 mM phosphate with a 20% acetonitrile at pH 7) is attributable to the micellar character of the MIP-NPs, provided by the inclusion of the SUG monomers in the structure. To our knowledge this is the first example of “monoclonal” MIP-NPs incorporated in CEC separations of drug enantiomers.Keywords
This publication has 37 references indexed in Scilit:
- Recent applications in capillary electrochromatographyElectrophoresis, 2006
- Glycine-based polymeric surfactants with varied polar head group: II. Chemical selectivity in micellar electrokinetic chromatography using linear solvation energy relationshipsElectrophoresis, 2005
- Glycine-based polymeric surfactants with varied polar head group: I. Synthesis, characterization, and application in micellar electrokinetic chromatographyElectrophoresis, 2005
- Analytical separations in open‐tubular capillary electrochromatographyElectrophoresis, 2003
- Advances in sol‐gel based columns for capillary electrochromatography: Sol‐gel open‐tubular columnsElectrophoresis, 2002
- Peer Reviewed: Molecular Micelles: Novel Pseudostationary Phases for CEAnalytical Chemistry, 2001
- Single Molecule Detection in Life SciencesSingle Molecules, 2000
- Polymeric Anionic Surfactant for Electrokinetic Chromatography: Separation of 16 Priority Polycyclic Aromatic Hydrocarbon PollutantsAnalytical Chemistry, 1998
- Study of the nature of recognition in molecularly imprinted polymersJournal of Molecular Recognition, 1996
- Emulsion polymerization: Initiation of polymerization in monomer dropletsJournal of Polymer Science Part C: Polymer Letters, 1973