Excitability of Skeletal Muscle during Development, Denervation, and Tissue Culture
- 1 October 2007
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering
- Vol. 13 (10), 2395-2404
- https://doi.org/10.1089/ten.2006.0367
Abstract
A quantitative understanding of the bulk excitability of skeletal muscle tissues is important for the design of muscle tissue bioreactor systems, implantable muscle stimulators, and other systems where electrical pulses are employed to elicit contractions in muscle tissue both in vitro and in vivo. The purpose of the present study is to systematically compare the excitability of mammalian (rat) skeletal muscle under a range of conditions (including neonatal development, denervation, and chronic in vivo stimulation of denervated muscle) and of self-organized muscle tissue constructs engineered in vitro from both primary cells and cell lines. Excitability is represented by rheobase (R50, units = V/mm) and chronaxie (C50, units = microseconds) values, with lower values for each indicating greater excitability. Adult skeletal muscle is the most excitable (R50 ∼ 0.29, C50 ∼ 100); chronically denervated whole muscles (R50 ∼ 2.54, C50 ∼ 690) and muscle engineered in vitro from cell lines (C2C12 + 10T1/2) (R50 ∼ 1.93, C50 ∼ 416) have exceptionally low excitability; muscle engineered in vitro from primary myocytes (R50 ∼ 0.99, C50 ∼ 496) has excitability similar to that of day 14 neonatal rat muscle (R50 ∼ 0.65, C50 ∼ 435); stimulated-denervated muscles retain excellent excitability when chronically electrically stimulated (R50 ∼ 0.40, C50 ∼ 100); and neonatal rat muscle excitability improves during the first 6 weeks of development, steadily approaching that of adult muscle.Keywords
This publication has 35 references indexed in Scilit:
- Determination of the Chronaxie and Rheobase of Denervated Limb Muscles in Conscious RabbitsArtificial Organs, 2005
- Number of contractions to maintain mass and force of a denervated rat muscleMuscle & Nerve, 2004
- Functional Development of Engineered Skeletal Muscle from Adult and Neonatal RatsTissue Engineering, 2001
- Power output of fast and slow skeletal muscles of mdx (dystrophic) and control mice after clenbuterol treatmentExperimental Physiology, 2000
- Evidence of inability to fully activate human limb muscleMuscle & Nerve, 2000
- Relations between excitability and contractility in rat soleus muscle: role of the Na+‐K+ pump and Na+/K+ gradientsThe Journal of Physiology, 1999
- Standing Up with Denervated Muscles in Humans Using Functional Electrical StimulationArtificial Organs, 1999
- THE STIMULATING EFFECT OF ELECTRIC CURRENTS OF EXTREMELY SHORT DURATIONImmunology & Cell Biology, 1947
- Muscle Changes in Denervation and Re-innervationBMJ, 1945
- THE ELECTRICAL REACTIONS OF MUSCLES BEFORE AND AFTER NERVE INJURYBrain, 1916