Cloacibacillus porcorum sp. nov., a mucin-degrading bacterium from the swine intestinal tract and emended description of the genus Cloacibacillus
Open Access
- 1 June 2013
- journal article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 63 (Pt_6), 1960-1966
- https://doi.org/10.1099/ijs.0.044719-0
Abstract
A novel anaerobic, mesophilic, amino-acid-fermenting bacterium, designated strain CL-84T, was isolated from the swine intestinal tract on mucin-based media. Cells were curved rods (0.8–1.2×3.5–5.0 µm), stained Gram-negative and were non-motile with no evidence of spores. Strain CL-84T produced acetate, propionate, formate and butyrate as the end products of metabolism when grown on serine. Optimum growth occurred at 39 °C and pH 6.5. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 and C16 : 0, distinguishing strain CL-84T from closely related species. The DNA G+C content of strain CL-84T was 55.1 mol%. 16S rRNA gene sequence analysis showed that strain CL-84T shared 90–95 % similarity with characterized genera within the phylum Synergistetes , family Synergistaceae . Phylogenetic analysis showed that strain CL-84T was related to, but distinct from, Cloacibacillus evryensis . Based on these findings, we propose that strain CL-84T represents a novel species of the genus Cloacibacillus . We further propose the name Cloacibacillus porcorum sp. nov. be designated for this species. The type strain is CL-84T ( = DSM 25858T = CCUG 62631T). An emended description of the genus Cloacibacillus is provided.Keywords
Funding Information
- Agricultural Research Service
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