Structural characterization of oligosaccharides by high-performance liquid chromatography, fast-atom bombardment-mass spectrometry, and exoglycosidase digestion
- 1 September 1984
- journal article
- research article
- Published by Elsevier BV in Analytical Biochemistry
- Vol. 141 (2), 366-381
- https://doi.org/10.1016/0003-2697(84)90057-5
Abstract
A method for structural characterization of oligosaccharides after prepararing UV-absorbing derivatives was described. The derivatives can be rapidly analyzed and purified by high-performance liquid chromatography, with separation of various structures determined primarily by size and sugar composition. Derivatization requires as little as 0.5-1.0 nmol of oligosaccharide and detection of down to 50 pmol of oligosaccharide is possible by monitoring absorbance at 229 nm. In addition, the carbohydrate portion of the derivative was found to retain its sensitivity to exoglycosidases, allowing sequential enzymatic digestions for determination of sugar sequence and anomerity to be performed. The derivatives also possessed a site of potential positive charge, making them amenable to analysis by fast-atom bombardment-mass spectrometry. Permethylation of the derivatives permitted their separation by capillary gas chromatography, thus allowing investigation of their structures by gas chromatography-mass spectrometry. The combination of these techniques will allow almost the complete structure of small amounts of oligosaccharides to be determined.This publication has 22 references indexed in Scilit:
- Fast atom bombardment mass spectrometry of carbohydrate chains derived from glycoproteinsJournal of Mass Spectrometry, 1983
- Tandem mass spectrometry (MS/MS) instrumentationMass Spectrometry Reviews, 1983
- New Techniques for Qligosaccharide SequencingJournal of Carbohydrate Chemistry, 1983
- New mass spectral approaches to complex carbohydrate structureMass Spectrometry Reviews, 1983
- Diethanolamine assisted secondary ion mass spectrometry of naturally occurring complex oligosaccharidesJournal of Mass Spectrometry, 1982
- Mass Spectrometry of Middle MoleculesAnalytical Chemistry, 1982
- Comparative study of [Xe]+ and [Ar]+ bombardment in secondary ion mass spectrometry for bioorganic compoundsJournal of Mass Spectrometry, 1982
- Quantitative microanalysis of oligosaccharides by high-performance liquid chromatographyCarbohydrate Research, 1981
- The structure of canine intestinal trihexosylceramideBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1979
- Structure analyses of oligosaccharides by tagging of the reducing end sugars with a fluorescent compoundBiochemical and Biophysical Research Communications, 1978