Contribution of genes from the capsule gene complex (cps) to lipooligosaccharide biosynthesis and serum resistance in Neisseria meningitidis
- 1 March 1994
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 11 (5), 885-896
- https://doi.org/10.1111/j.1365-2958.1994.tb00367.x
Abstract
Within the capsule gene complex (cps) of Neisseria meningitidis B a 5.5 kb DNA fragment encodes proteins with strong homologies to enzymes of the lipopolysaccharide biosynthetic pathway of Salmonella typhimurium and Escherichia coli, GalE, RfbB, RfbC and RfbD. A meningococcal galE mutant expressed a truncated lipooligosaccharide (LOS), which terminated at the glucose residue between inner and outer core, and a second gaiE gene present outside the cps cluster was found to be transcriptionally and functionally inactive and, thus, unable to complement this defect. Because of the defect in the outer core, the LOS of the galE‐defective meningococcal mutant was not sialylated. In contrast, carbohydrate analysis of the LOS of an rfb‐defective meningococcal mutant revealed no difference from the LOS of the wild‐type strain, suggesting that the rfb genes are inactive. This was supported by Northern blot analysis, which showed that expression of the rfb gene products was transcriptionally regulated. The inability of the meningococcal galE mutant, which cannot sialylate the LOS, allowed us to investigate the significance of LOS sialylation in relation to the presence of the polysialic acid capsule. Sialylated LOS, but not the polysialic acid capsule, is necessary to confer complete serum resistance on the meningococcus by inhibition of the alternative complement pathway.Keywords
This publication has 49 references indexed in Scilit:
- Cloning and molecular analysis of the galE gene of Neisseria meningitidls and its role in lipopolysaccharide biosynthesisMolecular Microbiology, 1993
- Genetic variation in pathogenic bacteriaTrends in Genetics, 1992
- Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2)Molecular Microbiology, 1991
- Structure of the L1 and L6 core oligosaccharide epitopes of NeisseriameningitidisCanadian Journal of Chemistry, 1990
- In vitro and in vivo modification of Neisseria gonorrhoeae lipooligosaccharide epitope structure by sialylation.The Journal of Experimental Medicine, 1990
- N-propionylated group B meningococcal polysaccharide mimics a unique epitope on group B Neisseria meningitidis.The Journal of Experimental Medicine, 1987
- ANTIGENIC SIMILARITIES BETWEEN BRAIN COMPONENTS AND BACTERIA CAUSING MENINGITISThe Lancet, 1983
- Differential Determination of the 3‐Deoxy‐d‐mannooctulosonic Acid Residues in Lipopolysaccharides of Salmonella minnesota Rough MutantsEuropean Journal of Biochemistry, 1983
- Clinical evaluation of group A and group C meningococcal polysaccharide vaccines in infants.JCI Insight, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970