Enantiomer resolution by using capillary zone electrophoresis: Resolution of racemic tryptophan and determination of the enantiomer composition of commercial pharmaceutical epinephrine

Abstract

The enantiomers of D- and L-tryptophan were separated by capillary electrophoresis, using α-cyclodextrin as a chiral active component in the background electrolyte. The separation of (-) and (+) epinephrine was achieved by supplementing the background electrolyte with Heptakis (2,6-di-O-methyl-β-cyclodextrin). As a practical application of the method, the quantitative analysis of (-)and(+)enantiomers in commercial pharmaceutical solutions of adrenaline is shown.