Digital Microfluidic Chip for Rapid Portable Detection of Mercury(II)
- 10 October 2011
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Sensors Journal
- Vol. 11 (11), 2820-2824
- https://doi.org/10.1109/jsen.2011.2170969
Abstract
This paper reports a rapid and visual detection of mercury ion on a new developed digital microfluidic chip with electrostatic manipulated droplets, which employed a principle of conjugated polymers and label-free oligonucleotides for mercury ion detection. The complex of water-soluble conjugated polymer, poly (3-(3' -N,N,N-triethylamino-1' -pro-pyloxy)-4-methyl-2,5-thiophene hydrochloride) (PMNT) and a label-free, mercury-specific oligonucleotide (MSO) probe is used as a mercury detector. While the detector droplet is fixed on the microfluidic chip, the target mercury ionic droplet is transported to the detector and then mixed together by electrostatic force driving, resulting in the color of the detector changing from red to yellow. We demonstrate that the detection system is portable and highly selective, and it can identify micromolar Hg2+ concentrations within minutes with the naked eye.This publication has 13 references indexed in Scilit:
- A Low Voltage Driven Digital-Droplet-Transporting-Chip by Electrostatic ForceChinese Physics Letters, 2011
- Microfluidic lab-on-a-chip platforms: requirements, characteristics and applicationsChemical Society Reviews, 2010
- Highly Sensitive Electrochemical Sensor for Mercury(II) Ions by Using a Mercury-Specific Oligonucleotide Probe and Gold Nanoparticle-Based AmplificationAnalytical Chemistry, 2009
- Rational Design of “Turn‐On” Allosteric DNAzyme Catalytic Beacons for Aqueous Mercury Ions with Ultrahigh Sensitivity and SelectivityAngewandte Chemie, 2007
- Microfluidic platforms for lab-on-a-chip applicationsLab on a Chip, 2007
- Determination of trace levels of mercury in aqueous solutions by inductively coupled plasma atomic emission spectrometry: Elimination of the ‘memory effect’Microchemical Journal, 2007
- Colorimetric Detection of Mercuric Ion (Hg2+) in Aqueous Media using DNA‐Functionalized Gold NanoparticlesAngewandte Chemie, 2007
- Optical Detection of Mercury(II) in Aqueous Solutions by Using Conjugated Polymers and Label‐Free OligonucleotidesAdvanced Materials, 2007
- Inorganic Mercury Inhibits the Activation of LAT in T-Cell Receptor-Mediated Signal TransductionToxicological Sciences, 2005
- Highly Selective Oligonucleotide‐Based Sensor for Mercury(II) in Aqueous SolutionsAngewandte Chemie, 2004