Water solution of onion crude powder inhibits RANKL-induced osteoclastogenesis through ERK, p38 and NF-κB pathways
- 28 May 2008
- journal article
- research article
- Published by Springer Science and Business Media LLC in Osteoporosis International
- Vol. 20 (1), 93-103
- https://doi.org/10.1007/s00198-008-0630-2
Abstract
Onion powder has been reported to decrease the ovariectomy-induced bone resorption of rats. However, the molecular mechanism of onion powder on the bone cells has not been reported. Here, we report that water solution of onion crude powder decreases the osteoclastogenesis from co-cultures of bone marrow stromal cells and macrophage cells. Additionally, water solution of onion crude powder inhibits the RANKL-induced ERK, p38 and NF-κB activation in macrophages. In summary, our data showed that onion powder may benefit bone through an anti-resorption effect on the osteoclasts. A nutritional approach is important for both prevention and treatment of osteoporosis. Onion has been reported to decrease the ovariectomy-induced bone resorption. However, the functional effects of onion on the cultured osteoclasts and osteoblasts remain largely unknown. Here, we found that water solution of onion crude powder markedly inhibited the receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclastogenesis through ERK, p38 and NF-κB pathways. Other studies were also designed to investigate the potential signaling pathways involved in onion-induced decrease in osteoclastogenesis. The osteoclastogenesis was examined using the TRAP staining method. The MAPKs and NF-κB pathways were measured using Western blot analysis. A transfection protocol was used to examine NF-κB activity. Water solution of onion crude powder inhibited the RANKL plus M-CSF-induced osteoclastic differentiation from either bone marrow stromal cells or from RAW264.7 macrophage cells. Treatment of RAW264.7 macrophages with RANKL could induce the activation of ERK, p38 and NF-κB that was inhibited by water solution of onion crude powder. On the other hand, it did not affect the cell proliferation and differentiation of human cultured osteoblasts. Our data suggest that water solution of onion crude powder inhibits osteoclastogenesis from co-cultures of bone marrow stromal cells and macrophage cells via attenuation of RANKL-induced ERK, p38 and NF-κB activation.Keywords
This publication has 38 references indexed in Scilit:
- Basic fibroblast growth factor stimulates fibronectin expression through phospholipase C γ, protein kinase C α, c‐Src, NF‐κB, and p300 pathway in osteoblastsJournal of Cellular Physiology, 2007
- Attenuation of Bone Mass and Increase of Osteoclast Formation in Decoy Receptor 3 Transgenic MicePublished by Elsevier BV ,2007
- Ultrasound Stimulates Cyclooxygenase-2 Expression and Increases Bone Formation through Integrin, Focal Adhesion Kinase, Phosphatidylinositol 3-Kinase, and Akt Pathway in OsteoblastsMolecular Pharmacology, 2006
- TeriparatideNature Reviews Drug Discovery, 2003
- Onion and a Mixture of Vegetables, Salads, and Herbs Affect Bone Resorption in the Rat by a Mechanism Independent of Their Base ExcessJournal of Bone and Mineral Research, 2002
- Nitric oxide and boneImmunology, 2001
- Rutin Inhibits Ovariectomy-Induced Osteopenia in RatsJournal of Bone and Mineral Research, 2000
- EditorialBone, 1999
- Characterization of the Intracellular Domain of Receptor Activator of NF-κB (RANK)Published by Elsevier BV ,1998
- The Cell-surface Form of Colony-stimulating Factor-1 Is Regulated by Osteotropic Agents and Supports Formation of Multinucleated Osteoclast-like CellsPublished by Elsevier BV ,1998