Conserved molecular portraits of bovine and human blastocysts as a consequence of the transition from maternal to embryonic control of gene expression
- 1 October 2007
- journal article
- research article
- Published by American Physiological Society in Physiological Genomics
- Vol. 31 (2), 315-327
- https://doi.org/10.1152/physiolgenomics.00041.2007
Abstract
The present study investigated mRNA expression profiles of bovine oocytes and blastocysts by using a cross-species hybridization approach employing an array consisting of 15,529 human cDNAs as probe, thus enabling the identification of conserved genes during human and bovine preimplantation development. Our analysis revealed 419 genes that were expressed in both oocytes and blastocysts. The expression of 1,324 genes was detected exclusively in the blastocyst, in contrast to 164 in the oocyte including a significant number of novel genes. Genes indicative for transcriptional and translational control (ELAVL4, TACC3) were overexpressed in the oocyte, whereas cellular trafficking (SLC2A14, SLC1A3), proteasome (PSMA1, PSMB3), cell cycle (BUB3, CCNE1, GSPT1), and protein modification and turnover (TNK1, UBE3A) genes were found to be overexpressed in blastocysts. Transcripts implicated in chromatin remodeling were found in both oocytes (NASP, SMARCA2) and blastocysts (H2AFY, HDAC7A). The trophectodermal markers PSG2 and KRT18 were enriched 5- and 50-fold in the blastocyst. Pathway analysis revealed differential expression of genes involved in 107 distinct signaling and metabolic pathways. For example, phosphatidylinositol signaling and gluconeogenesis were prominent pathways identified in the blastocyst. Expression patterns in bovine and human blastocysts were to a large extent identical. This analysis compared the transcriptomes of bovine oocytes and blastocysts and provides a solid foundation for future studies on the first major differentiation events in blastocysts and identification of a set of markers indicative for regular mammalian development.Keywords
This publication has 73 references indexed in Scilit:
- The transcriptome of human oocytesProceedings of the National Academy of Sciences of the United States of America, 2006
- The role of ETS transcription factors in transcription and development of mouse preimplantation embryosBiochemical and Biophysical Research Communications, 2006
- Evidence that Poly(A) Binding Protein C1 Binds Nuclear Pre-mRNA Poly(A) TailsMolecular and Cellular Biology, 2006
- The human cumulus–oocyte complex gene-expression profileHuman Reproduction, 2006
- Variants in Deleted in AZoospermia-Like (DAZL) are correlated with reproductive parameters in men and womenHuman Genetics, 2005
- Identification of Novel and Known Oocyte-Specific Genes Using Complementary DNA Subtraction and Microarray Analysis in Three Different Species1Biology of Reproduction, 2005
- SWI/SNF Chromatin-Remodeling Factors Induce Changes in DNA Methylation to Promote Transcriptional ActivationCancer Research, 2005
- Transgenic RNAi-mediated reduction of MSY2 in mouse oocytes results in reduced fertilityDevelopmental Biology, 2004
- Poly(A) length, cytoplasmic adenylation and synthesis of poly(A)+ RNA in early mouse embryosDevelopmental Biology, 1983
- Robust Locally Weighted Regression and Smoothing ScatterplotsJournal of the American Statistical Association, 1979