Novel Type of Staphylococcal Cassette Chromosome mec in a Methicillin-Resistant Staphylococcus aureus Strain Isolated in Sweden
Open Access
- 1 October 2008
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 52 (10), 3512-3516
- https://doi.org/10.1128/aac.00087-08
Abstract
We identified a novel type of staphylococcal cassette chromosome mec (SCC mec ) element carried by methicillin-resistant Staphylococcus aureus (MRSA) strain JCSC6082 isolated in Sweden. The SCC mec element was demarcated by characteristic nucleotide sequences at both ends and was integrated at the 3′ end of orfX . The element carried a novel combination of a type 5 ccr gene complex and class C1 mec gene complex. The J regions of the element were homologous to those of the SCC mercury element of S. aureus strain 85/2082, with nucleotide identity greater than 99%. However, the novel SCC mec element from JCSC6082 did not carry the mer operon nor Tn 554 , suggesting that evolution to SCC mec could have been from a common ancestor by acquisition of the class C1 mec gene complex. The novel SCC mec element from JCSC6082 was flanked by a novel SCC-like chromosome cassette (CC6082), which was demarcated by two direct repeats and could be excised from the chromosome independently of the SCC mec element. Our data suggest that novel SCC mec elements can be generated on the staphylococcal chromosome through the recombination between extant SCC elements and mec gene complexes.This publication has 19 references indexed in Scilit:
- Novel Characteristics of Community-Acquired Methicillin-Resistant Staphylococcus aureus Strains Belonging to Multilocus Sequence Type 59 in TaiwanAntimicrobial Agents and Chemotherapy, 2008
- Combination of Multiplex PCRs for Staphylococcal Cassette Chromosome mec Type Assignment: Rapid Identification System for mec , ccr , and MajorDifferences in Junkyard RegionsAntimicrobial Agents and Chemotherapy, 2007
- Predominance of Clones Carrying Panton-Valentine Leukocidin Genes among Methicillin-Resistant Staphylococcus aureus Strains Isolated in Japanese Hospitals from 1979 to 1985Journal of Clinical Microbiology, 2006
- Staphylococcal Cassette Chromosome mec (SCC mec ) Typing of Methicillin-Resistant Staphylococcus aureus Strains Isolated in 11 Asian Countries: a Proposal for a New Nomenclature for SCC mec ElementsAntimicrobial Agents and Chemotherapy, 2006
- Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureusThe Lancet, 2006
- Multilocus Sequence Typing of Methicillin-Resistant Staphylococcus aureus from an Area of Low Endemicity by Real-Time PCRJournal of Clinical Microbiology, 2005
- Predominance of staphylococcal cassette chromosome mec (SCCmec) type IV among methicillin-resistant Staphylococcus aureus (MRSA) in a Swedish county and presence of unknown SCCmec types with Panton-Valentine leukocidin genesClinical Microbiology & Infection, 2005
- Novel Type V Staphylococcal Cassette Chromosome mec Driven by a Novel Cassette Chromosome Recombinase, ccrCAntimicrobial Agents and Chemotherapy, 2004
- Dissemination of New Methicillin-Resistant Staphylococcus aureus Clones in the CommunityJournal of Clinical Microbiology, 2002
- Cloning and Nucleotide Sequence Determination of the Entire mec DNA of Pre-Methicillin-Resistant Staphylococcus aureus N315Antimicrobial Agents and Chemotherapy, 1999