PCR Detection Method ofClostridium scindensandC. hiranonisin Human Fecal Samples
Open Access
- 1 March 2001
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 45 (3), 263-266
- https://doi.org/10.1111/j.1348-0421.2001.tb02616.x
Abstract
Clostridium scindens and C. hiranonis have high bile acid 7α‐dehydroxylating activity. Direct PCR and nested PCR with the specific primers for C. scindens and C. hiranonis were developed and tested for the detection of them in human fecal samples. Nested PCR detected C. scindens in all samples, but direct PCR detected it in 27 of 34 samples. Regarding C. hiranonis, nested PCR detected it in only 18 of 34 samples although nested PCR has a high level of sensitivity. Nested PCR was a specific and sensitive method for the detection of C. scindens and C. hiranonis in fecal samples.Keywords
This publication has 8 references indexed in Scilit:
- Clostridium hiranonis sp. nov., a human intestinal bacterium with bile acid 7alpha-dehydroxylating activity.International Journal of Systematic and Evolutionary Microbiology, 2001
- Assignment of Eubacterium sp. VPI 12708 and related strains with high bile acid 7alpha-dehydroxylating activity to Clostridium scindens and proposal of Clostridium hylemonae sp. nov., isolated from human faeces.International Journal of Systematic and Evolutionary Microbiology, 2000
- Isolation and characterization of cholic acid 7α-dehydroxylating fecal bacteria from cholesterol gallstone patientsJournal of Hepatology, 2000
- Isolation and Characterization of Bile Acid 7‐Dehydroxylating Bacteria from Human FecesMicrobiology and Immunology, 1995
- Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chlorideNucleic Acids Research, 1993
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Transformation of cholic acid by Clostridium bifermentansJournal of Applied Bacteriology, 1982
- 7α‐dehydroxylation of cholic acid by Clostridium bifermentans strain ATCC 9714 and Clostridium sordellii strain NCIB 6929FEBS Letters, 1970