Overexpression of an mRNA-binding protein in human colorectal cancer
- 4 October 2001
- journal article
- case report
- Published by Springer Science and Business Media LLC in Oncogene
- Vol. 20 (45), 6544-6550
- https://doi.org/10.1038/sj.onc.1204838
Abstract
A Coding Region instability Determinant-Binding Protein (CRD-BP) binds in vitro to c-myc mRNA and appears to stabilize the mRNA. The CRD-BP gene is amplified in one-third of human breast cancer cases, and the CRD-BP appears to be an oncofetal protein. To analyse CRD-BP expression in human cancer tissue, paired extracts of cancer and normal colon specimens from 21 patients were analysed by immunoblotting and/or reverse transcriptase-polymerase chain reaction. Seventeen cancer specimens out of 21 (81%) were positive for CRD-BP expression by one or both assays. With one exception, normal colon specimens were negative for CRD-BP expression; specimens of inflammatory bowel and a villous adenoma also had no detectable CRD-BP. The lack of CRD-BP expression in normal colon did not result from indiscriminate protein or RNA degradation. c-myc mRNA levels appeared to be elevated in tumor specimens. We conclude that the CRD-BP is scarce or absent from normal colon but is overexpressed in colorectal cancer. The CRD-BP might be a novel human tumor marker.Keywords
This publication has 21 references indexed in Scilit:
- American Joint Committee on Cancer prognostic factors consensus conferenceCancer, 2000
- The c-myc coding region determinant-binding protein: a member of a family of KH domain RNA-binding proteinsNucleic Acids Research, 1998
- Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reactionBritish Journal of Cancer, 1998
- RNA-binding protein conserved in both microtubule- and microfilament-based RNA localizationGenes & Development, 1998
- A highly conserved RNA-binding protein for cytoplasmic mRNA localization in vertebratesCurrent Biology, 1998
- Purification of Total RNA from Human Stool SamplesDigestive Diseases and Sciences, 1998
- Lessons from Hereditary Colorectal CancerCell, 1996
- The half-life of c-myc mRNA in growing and serum-stimulated cells: influence of the coding and 3' untranslated regions and role of ribosome translocation.Molecular and Cellular Biology, 1994
- Control of c-myc mRNA half-life in vitro by a protein capable of binding to a coding region stability determinant.Genes & Development, 1992
- Expression of c-myc oncogene in colorectal polyps as a biological marker for monitoring malignant potentialCancer, 1989