Isolation and characterization of microparticles in sputum from cystic fibrosis patients
Open Access
- 9 July 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Respiratory Research
- Vol. 11 (1), 94
- https://doi.org/10.1186/1465-9921-11-94
Abstract
Microparticles (MPs) are membrane vesicles released during cell activation and apoptosis. MPs have different biological effects depending on the cell from they originate. Cystic fibrosis (CF) lung disease is characterized by massive neutrophil granulocyte influx in the airways, their activation and eventually apoptosis. We investigated on the presence and phenotype of MPs in the sputum, a rich non-invasive source of inflammation biomarkers, of acute and stable CF adult patients. Spontaneous sputum, obtained from 21 CF patients (10 acute and 11 stable) and 7 patients with primary ciliary dyskinesia (PCD), was liquefied with Sputasol. MPs were counted, visualized by electron microscopy, and identified in the supernatants of treated sputum by cytofluorimetry and immunolabelling for leukocyte (CD11a), granulocyte (CD66b), and monocyte-macrophage (CD11b) antigens. Electron microscopy revealed that sputum MPs were in the 100-500 nm range and did not contain bacteria, confirming microbiological tests. CF sputa contained higher number of MPs in comparison with PCD sputa. Levels of CD11a+-and CD66b+-, but not CD11b+-MPs were significantly higher in CF than in PCD, without differences between acute and stable patients. In summary, MPs are detectable in sputa obtained from CF patients and are predominantly of granulocyte origin. This novel isolation method for MPs from sputum opens a new opportunity for the study of lung pathology in CF.Keywords
This publication has 48 references indexed in Scilit:
- Parasite-Derived Plasma Microparticles Contribute Significantly to Malaria Infection-Induced Inflammation through Potent Macrophage StimulationPLoS Pathogens, 2010
- Procoagulant alveolar microparticles in the lungs of patients with acute respiratory distress syndromeAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2009
- Dysfunctional cystic fibrosis transmembrane conductance regulator inhibits phagocytosis of apoptotic cells with proinflammatory consequencesAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2009
- microRNAs and the immune responseCurrent Opinion in Pharmacology, 2009
- Activation of critical, host-induced, metabolic and stress pathways marks neutrophil entry into cystic fibrosis lungsProceedings of the National Academy of Sciences of the United States of America, 2009
- Detection of microRNA Expression in Human Peripheral Blood MicrovesiclesPLOS ONE, 2008
- Sputum Proteomics in Inflammatory and Suppurative Respiratory DiseasesAmerican Journal of Respiratory and Critical Care Medicine, 2008
- Profound functional and signaling changes in viable inflammatory neutrophils homing to cystic fibrosis airwaysProceedings of the National Academy of Sciences of the United States of America, 2008
- Function and role of microparticles in various clinical settingsThrombosis Research, 2008
- Association between Pulmonary Function and Sputum Biomarkers in Cystic FibrosisAmerican Journal of Respiratory and Critical Care Medicine, 2007