Non‐Fouling Biodegradable Poly(ϵ‐caprolactone) Nanofibers for Tissue Engineering
- 7 October 2015
- journal article
- research article
- Published by Wiley in Macromolecular Bioscience
- Vol. 16 (1), 83-94
- https://doi.org/10.1002/mabi.201500252
Abstract
Poly(ϵ‐caprolactone) (PCL) nanofibers are very attractive materials for tissue engineering (TE) due to their degradability and structural similarity to the extracellular matrix (ECM). However, upon exposure to biological media, their surface is rapidly fouled by proteins and cells, which may lead to inflammation and foreign body reaction. In this study, an approach for the modification of PCL nanofibers to prevent protein fouling from biological fluids and subsequent cell adhesion is introduced. A biomimetic polydopamine (PDA) layer was deposited on the surface of the PCL nanofibers and four types of antifouling polymer brushes were grown by surface‐initiated atom transfer radical polymerization (SI‐ATRP) from initiator moieties covalently attached to the PDA layer. Cell adhesion was assessed with mouse embryonic fibroblasts (MEFs). MEFs rapidly adhered and formed cell–matrix adhesions (CMAs) with PCL and PCL‐PDA nanofibers. Importantly, the nanofibers modified with antifouling polymer brushes were able to suppress non‐specific protein adsorption and thereby cell adhesion.Funding Information
- Grant Agency of the Czech Republic (GACR) (15-09368Y)
- “BIOCEV - Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University” (CZ.1.05/1.1.00/02.0109)
- Ministry of Education, Youth and Sports of the Czech Republic (10/297/OHK4/3T/14LH13178)
- Karlsruhe School of Optics and Photonics KSOP
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