Enumeration of Viable Listeria monocytogenes Cells by Real-Time PCR with Propidium Monoazide and Ethidium Monoazide in the Presence of Dead Cells
- 15 December 2007
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (24), 8028-8031
- https://doi.org/10.1128/aem.01198-07
Abstract
Propidium monoazide (PMA) and ethidium monoazide were used for enumeration of viable Listeria monocytogenes cells in the presence of dead cells. PMA had no antimicrobial effect on L. monocytogenes . Viable cell counts were linearly related to real-time PCR threshold cycle values for PMA-treated cells from planktonic and biofilm sources over a 4-log range.Keywords
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