Behavior ofPseudomonas fluorescens within the hydrodynamic boundary layers of surface microenvironments
- 1 July 1987
- journal article
- research article
- Published by Springer Science and Business Media LLC in Microbial Ecology
- Vol. 14 (1), 1-14
- https://doi.org/10.1007/bf02011566
Abstract
Phase, darkfield, and computer-enhanced microscopy were used to observe the surface microenvironment of flow cells during bacterial colonization. Microbial behavior was consistent with the assumptions used previously to derive surface colonization kinetics and to calculate surface growth and attachment rates from cell number and distribution. Surface microcolonies consisted of closely packed cells. Each colony contained 2n cells, where n is the number of cell divisions following attachment. Initially, cells were freely motile while attached, performing circular looping movements within the plane of the solid-liquid interface. Subsequently, cells attached apically, maintained a fixed position on the surface, and rotated. This type of attachment was reversible and did not necessarily lead to the formation of microcolonies. Cells became irreversibly attached by progressing from apical to longitudinal attachment. Longitudinally attached cells increased in length, then divided, separated, moved apart laterally, and slid next to one another. This resulted in tight cell packing and permitted simultaneous growth and adherence. After approximately 4 generations, individual cells emigrated from developing microcolonies to recolonize the surface at new locations. Surface colonization byPseudomonas fluorescens can thus be subdivided into the following sequential colonization phases: motile attachment phase, reversible attachment phase, irreversible attachment phase, growth phase, and recolonization phase.Keywords
This publication has 38 references indexed in Scilit:
- Behavior of bacterial stream populations within the hydrodynamic boundary layers of surface microenvironmentsMicrobial Ecology, 1987
- Growth kinetics ofPseudomonas fluorescens microcolonies within the hydrodynamic boundary layers of surface microenvironmentsMicrobial Ecology, 1986
- Evaluation of surface colonization kinetics in continuous cultureMicrobial Ecology, 1983
- A computer simulation of surface microcolony formation during microbial colonizationMicrobial Ecology, 1983
- Derivation of a growth rate equation describing microbial surface colonizationMicrobial Ecology, 1983
- Evaluation of a proposed surface colonization equation usingThermothrix thiopara as a model organismMicrobial Ecology, 1982
- Quantitation of microbial growth on surfacesMicrobial Ecology, 1981
- An ecological study of the sulfur-oxidizing bacteria from the littoral zone of a michigan lake and a sulfur spring in FloridaPlant and Soil, 1975
- Mechanism of the Initial Events in the Sorption of Marine Bacteria to SurfacesJournal of General Microbiology, 1971
- GROWTH AND METABOLISM OF PERIPHYTIC BACTERIA: METHODOLOGYLimnology and Oceanography, 1970